Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae has been associated with a wide range of infections in humans and animals. The objective of this study was to determine the genomic characteristics of two multiple drug resistant, ESBLs-producing K. pneumoniae strains isolated from a swine in 2013 (KP2013Z28) and a hospitalized patient in 2014 (KP2014C46) in Malaysia. Genomic analyses of the two K. pneumoniae strains indicated the presence of various antimicrobial resistance genes associated with resistance to β-lactams, aminoglycosides, colistin, fluoroquinolones, phenicols, tetracycline, sulfonamides, and trimethoprim, corresponding to the antimicrobial susceptibility profiles of the strains. KP2013Z28 (ST25) and KP2014C46 (ST929) harbored 5 and 2 genomic plasmids, respectively. The phylogenomics of these two Malaysian K. pneumoniae, with other 19 strains around the world was determined based on SNPs analysis. Overall, the strains were resolved into five clusters that comprised of strains with different resistance determinants. This study provided a better understanding of the resistance mechanisms and phylogenetic relatedness of the Malaysian strains with 19 strains isolated worldwide. This study also highlighted the needs to monitor the usage of antibiotics in hospital settings, animal husbandry, and agricultural practices due to the increase of β-lactam, aminoglycosides, tetracycline, and colistin resistance among pathogenic bacteria for better infection control.

产超广谱 β-内酰胺酶 (ESBL) 的肺炎克雷伯菌与人类和动物的多种感染有关。本研究的目的是确定2013 年从一头猪 (KP2013Z28) 和 2014 年马来西亚住院患者 (KP2014C46) 中分离出的两种多重耐药、产 ESBL肺炎克雷伯菌菌株的基因组特征。两种肺炎克雷伯菌的基因组分析菌株表明存在与对 β-内酰胺类、氨基糖苷类、粘菌素、氟喹诺酮类、苯酚、四环素、磺胺类和甲氧苄啶的耐药性相关的各种抗菌素耐药基因，这与菌株的抗菌药物敏感性谱相对应。KP2013Z28 (ST25) 和 KP2014C46 (ST929) 分别含有 5 个和 2 个基因组质粒。这两种马来西亚肺炎克雷伯菌的系统基因组学，与世界各地的其他 19 个菌株是基于 SNP 分析确定的。总体而言，菌株被分解为五个簇，由具有不同抗性决定因素的菌株组成。这项研究提供了对马来西亚菌株的抗性机制和系统发育相关性的更好理解，其中包括全球分离的 19 株菌株。该研究还强调，由于致病菌对 β-内酰胺、氨基糖苷类、四环素和粘菌素的耐药性增加，因此需要监测医院环境、畜牧业和农业实践中抗生素的使用情况，以更好地控制感染。