Vibriosis is one of the common diseases caused by gram-negative bacteria from the genus Vibrio. To treat vibriosis, vaccination has been proven to be the most effective treatment as it can avoid the risk of drugs or antibiotics resistance. Microalgae are commonly used as feed for aquatic organisms and Nannochloropsis sp. is one of the highly utilized species for fish feed. This study focused on the use of microalga, Nannochloropsis sp. as a vaccine carrier. Transgenic Nannochloropsis sp. harbouring an outer membrane protein kinase (OmpK) gene fragment of the Vibrio species namely V1, V2, CV1, CV2, CPV1 and CPV2 were utilized in this study. The stability of OmpK gene in transgenic Nannochloropsis sp. over a number of generations was evaluated. DNA and RNA from the Nannochloropsis sp. transgenic lines were extracted and subjected to PCR amplification of OmpK gene fragment. The OmpK gene fragment was successfully amplified and expressed up to the fifth generation (F5). For V1, V2, CV1 and CV2, the gene was present and expressed in fourth generation (F4) and F5 respectively but CPV1 and CPV2 the OmpK genes were present up to F4. From the results obtained, Nannochloropsis sp. is shown to be suitable as a vaccine carrier and can be utilized as a vaccine carrier to ameliorate vibriosis.

弧菌病是弧菌属革兰氏阴性细菌引起的常见疾病之一。为了治疗弧菌病，疫苗接种已被证明是最有效的治疗方法，因为它可以避免药物或抗生素耐药的风险。微藻通常用作水生生物和拟南芥的饲料。是鱼类饲料中利用率最高的物种之一。这项研究的重点是微藻Nannochloropsis sp。的使用。作为疫苗载体。转基因Nannochloropsis sp。本研究利用了带有弧菌物种的外膜蛋白激酶（OmpK）基因片段，即V1，V2，CV1，CV2，CPV1和CPV2。OmpK的稳定性转基因Nannochloropsis sp。中的基因 经过几代人的评估。来自Nannochloropsis sp。的DNA和RNA 。提取转基因品系并进行OmpK基因片段的PCR扩增。所述OmpK基因片段成功扩增并表达到第五代（F5）。对于V1，V2，CV1和CV2，存在该基因并分别在第四代（F4）和F5中表达，但是CPV1和CPV2的OmpK基因存在直至F4。从获得的结果，Nannochloropsis sp。已显示出其适合用作疫苗载体并且可用作疫苗载体以改善弧菌病。