Human cervical cancer is the most common gynecological malignancy. The continuous development of nanotechnology has allowed the wide use of nanomaterials in cancer treatment. Nanoparticles can be used as gene carriers because of their surface effect and small-size effect. MicroRNA-let-7c-5p (miR-let-7c-5p) belongs to the let-7 family. Although it has been reported to exert a tumor suppressive effect in a variety of cancers, the exact role and mechanism of miR-let-7c-5p in the progression of cervical cancer are unclear. In this study, we synthesized flower-shaped SiO₂ -PEI nanoparticles with high pDNA/siRNA loading rates. This nanoparticle with miR-let-7c-5p-expressed plasmid could effectively transfer miR-let-7c-5p to human epithelial carcinoma (HeLa) cells. In addition, the combination of nanomaterials and gene therapy could inhibit the development of cancer under the conditions of extremely low cytotoxicity. These findings provided a new anticancer strategy based on F-SiO₂ -polyethyleneimine/miR-let-7c-5p (FSP-let-7c-5p)nanoparticles and indicated that miR-let-7c-5p/IGF-1R/PI3K/AKT and -catenin/SLUG could be used as new potential targets for the treatment of cervical cancer.

中文翻译：

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通过可生物降解的二氧化硅纳米粒子传递MicroRNA-let-7c-5p抑制人类宫颈癌细胞的增殖和迁移。

人宫颈癌是最常见的妇科恶性肿瘤。纳米技术的不断发展使纳米材料在癌症治疗中得到了广泛的应用。纳米颗粒由于其表面效应和小尺寸效应而可用作基因载体。MicroRNA-let-7c-5p（miR-let-7c-5p）属于let-7家族。尽管据报道在多种癌症中均具有抑癌作用，但尚不清楚miR-let-7c-5p在宫颈癌进展中的确切作用和机制。在这项研究中，我们合成了具有高pDNA / siRNA加载速率的花形SiO 2 -PEI纳米颗粒。具有表达miR-let-7c-5p的质粒的纳米粒子可以有效地将miR-let-7c-5p转移到人上皮癌（HeLa）细胞。此外，纳米材料与基因疗法的结合可以在极低的细胞毒性条件下抑制癌症的发展。这些发现提供了一种基于F-SiO 2-聚乙烯亚胺/ miR-let-7c-5p（FSP-let-7c-5p）纳米粒子的新抗癌策略，并表明miR-let-7c-5p / IGF-1R / PI3K / AKT -catenin / SLUG可作为治疗宫颈癌的新潜在靶点。