Salvia macrosiphon Boiss. is an aromatic perennial herb belonging to the family Lamiaceae. Phytochemical studies and biological activities of this plant have been rarely documented in the literature. The current study aimed to investigate antibacterial and cytotoxic activity of different fractions of aerial parts of S. macrosiphon. Also, we tried to isolate and identify cytotoxic compounds from the plant. In this respect, the hydroalcoholic extract of the corresponding parts of the plant was fractionated into four fractions. Then, antibacterial and cytotoxic activity of each fraction were examined. It was found that the chloroform fraction had a good antibacterial activity against gram-positive and gram-negative bacteria. The most potent cytotoxicity was also obtained by the n-hexane fraction comparing with etoposide as the reference drug which was selected for the study and characterization of secondary metabolites. Accordingly, 13-epi manoyl oxide (1), 6α-hydroxy-13-epimanoyl oxide (2), 5-hydroxy-7,4'-dimethoxyflavone (3), and β-sitosterol (4) were isolated and evaluated for their cytotoxic activity. Among them, compound 1 revealed significant cytotoxicity against A549, MCF-7, and MDA-MB-231. It merits mentioning that it showed high selectivity index ratio regarding the low cytotoxic effects on Human Dermal Fibroblast which can be considered as a promising anticancer candidate.

中文翻译：

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大叶丹参的植物化学成分和生物学活性。

丹参macrosiphon Boiss。是属于唇形科的一种多年生芳香植物。该植物的植物化学研究和生物学活性很少在文献中记载。当前的研究旨在研究大虹吸管空中部分不同部分的抗菌和细胞毒性活性。另外，我们尝试从植物中分离和鉴定细胞毒性化合物。在这方面，将植物的相应部分的水醇提取物分为四个部分。然后，检查每个部分的抗菌和细胞毒性活性。发现氯仿级分对革兰氏阳性和革兰氏阴性细菌具有良好的抗菌活性。与依托泊苷作为参考药物相比，正己烷馏分也获得了最有效的细胞毒性，依托泊苷被选作研究和表征次生代​​谢产物。因此，分离出13-表氧化环氧丙烷（1），6α-羟基-13-环氧丙烷（2），5-羟基-7,4'-二甲氧基黄酮（3）和β-谷甾醇（4）并对其进行评估。细胞毒活性。其中，化合物1显示出对A549，MCF-7和MDA-MB-231的明显细胞毒性。值得一提的是，它对人皮肤成纤维细胞的细胞毒性低，显示出较高的选择性指数比，可以认为是有希望的抗癌候选物。分离出β-谷甾醇和β-谷甾醇（4）并评估其细胞毒活性。其中，化合物1显示出对A549，MCF-7和MDA-MB-231的明显细胞毒性。值得一提的是，它对人真皮成纤维细胞的细胞毒性作用低，显示出较高的选择性指数比，可以认为是有希望的抗癌候选物。分离出β-谷甾醇和β-谷甾醇（4）并评估其细胞毒活性。其中，化合物1显示出对A549，MCF-7和MDA-MB-231的明显细胞毒性。值得一提的是，它对人皮肤成纤维细胞的细胞毒性低，显示出较高的选择性指数比，可以认为是有希望的抗癌候选物。