Patch-clamp technique is the gold standard for cellular electrophysiological measurements, which is capable of measuring single ion transport events across the cell membrane. However, the measurement possesses significant complexities, and it requires a high level of expertise, while its experimental throughput is nevertheless considerably low. Here, we suggest and experimentally demonstrate a laser-assisted method for performing cellular electrophysiological measurements. Femtosecond laser pulses, coupled to an optical microscope, are used to form a sub-micrometer hole on a thin polymer membrane separating two electrodes, where a nearby cell is subsequently placed onto the hole by negative pressure. Afterwards, the cell is punctured using subsequent laser exposure, revealing the cell membrane over the hole for electrophysiological recording. This system could be used to increase the output amount of the electrophysiological measurements substantially.

中文翻译：

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激光辅助细胞电生理测量系统

膜片钳技术是细胞电生理测量的黄金标准，它能够测量跨细胞膜的单个离子传输事件。然而，测量具有显着的复杂性，需要高水平的专业知识，而其实验吞吐量却相当低。在这里，我们建议并通过实验证明了一种用于进行细胞电生理测量的激光辅助方法。飞秒激光脉冲与光学显微镜耦合，用于在分隔两个电极的聚合物薄膜上形成亚微米孔，随后通过负压将附近的细胞置于孔上。然后，使用随后的激光照射刺穿细胞，露出孔上的细胞膜以进行电生理记录。