Biobutanol can be produced by Clostridia via an acetone–butanol–ethanol (ABE) fermentation under strictly anaerobic conditions. Oxygen-free nitrogen (OFN) gas is typically used to create anaerobic conditions for ABE fermentations. However, this method is not appropriate for large-scale fermentations as it is quite costly. The aim of this work was to study the feasibility of butanol production from sweet sorghum juice (SSJ) by Clostridium beijerinckii TISTR 1461 using various methods to create anaerobic conditions, i.e., growth of a strictly aerobic bacterium, an Arthrobacter sp., under different conditions and a chemical method using sodium dithionite (SDTN) to consume residual oxygen. SSJ containing 60 g/L of total sugar supplemented with 1.27 g/L of (NH₄)₂SO₄ was used as a substrate for butanol production. The results showed that 0.25 mM SDTN could create anaerobic conditions, but in this case, C.beijerinckii TISTR 1461 could produce butanol at a concentration (P_B) of only 8.51 g/L with a butanol productivity (Q_B) of 0.10 g/L·h. Arthrobacter sp. BCC 72131 could also be used to create anaerobic conditions. Mixed cultures of C.beijerinckii TISTR 1461 and Arthrobacter sp. BCC 72131 created anaerobic conditions by inoculating the C.beijerinckii 4 h after Arthrobacter. This gave a P_B of 10.39 g/L with a Q_B of 0.20 g/L·h. Comparing butanol production with the control treatment (using OFN gas to create anaerobic conditions, yielding a P_B of 9.88 g/L and Q_B of 0.21 g/L·h) indicated that using Arthrobacter sp. BCC 72131 was an appropriate procedure for creating anaerobic conditions for high levels of butanol production by C. beijerinckii TISTR 1461 from a SSJ medium.

中文翻译：

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使用节杆菌属细菌的新方法。为拜氏梭菌从甜高粱汁中生产生物丁醇创造厌氧条件

梭菌可以在严格的厌氧条件下通过丙酮-丁醇-乙醇（ABE）发酵生产生物丁醇。无氧氮气（OFN）气体通常用于为ABE发酵创造厌氧条件。但是，这种方法不适合大规模发酵，因为它的成本很高。这项工作的目的是研究使用多种方法产生厌氧条件，即由严格的需氧细菌，一种节杆菌属细菌，在不同条件下生长，由拜氏梭菌TISTR 1461通过甜高粱汁（SSJ）生产丁醇的可行性。化学方法是使用连二亚硫酸钠（SDTN）消耗残余的氧气。SSJ包含60 g / L的总糖和1.27 g / L的（NH ₄）₂ SO ₄用作丁醇生产的底物。结果表明，0.25毫SDTN可以创建厌氧条件，但在这种情况下，C.拜氏梭菌TISTR 1461可以在一个浓度（产生丁醇P_乙仅8.51克/ L与丁醇生产率的）（Q_乙0.10）克/ L·h。节藻。BCC 72131也可用于创建厌氧条件。C. beijerinckii TISTR 1461和Arthrobacter sp。的混合培养物。BCC 72131接种所产生的厌氧条件下拜氏梭菌4小时后节。这给了P _B为10.39 g / L，Q _B为0.20 g / L·h。将丁醇生产与对照处理（使用OFN气体创建厌氧条件，产生的P _B为9.88 g / L和Q _B为0.21 g / L·h）进行比较表明，使用了节杆菌。BCC 72131是一种合适的程序，可为厌氧条件下的拜氏梭菌TISTR 1461从SSJ培养基中大量生产丁醇创造条件。