Oxidative stress causes several chronic diseases including cancer. Some chemotherapeutic agents are not selective against tumor cells, causing oxidative stress in non-tumor cells. This study aimed to evaluate the cytotoxic effect of acetone extract of Ficus crocata(Miq.) Mart. ex Miq. (F. crocata) leaves (Ace-EFc) on cervical cancer cells, as well as its protective effect on hydrogen peroxide (H₂O₂)-induced lipoperoxidation and cytotoxicity in non-tumor HaCaT cells. Antioxidant activity was determined using the DPPH and ABTS radicals. Cell viability and lipoperoxidation were determined with MTT and 1-methyl-2-phenylindole assays, respectively. A model of H₂O₂-induced cytotoxicity and oxidative damage in HaCaT cells was established. HaCaT cells were exposed to the extract before or after exposure to H₂O₂, and oxidative damage and cell viability were evaluated. Ace-EFc inhibited the DPPH and ABTS radicals and showed a cytotoxic effect on SiHa and HeLa cells. Furthermore, the extract treatment had a protective effect on hydrogen peroxide-induced lipoperoxidation and cytotoxicity, avoiding the increase in MalonDiAldehyde (MDA) levels and the decrease in cell viability (p < 0.001). These results suggest that the metabolites of F. crocata leaves possess antioxidant and cytoprotective activity against oxidative damage. Thus, they could be useful for protecting cells from conditions that cause oxidative stress.

中文翻译：

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番红花提取物对宫颈癌细胞的细胞毒性及对过氧化氢诱导的HaCaT非肿瘤细胞氧化应激的保护作用

氧化应激会导致多种慢性疾病，包括癌症。一些化学治疗剂对肿瘤细胞没有选择性，从而在非肿瘤细胞中引起氧化应激。这项研究旨在评估番红花市场的丙酮提取物的细胞毒性作用。前Miq。（番红花）叶（Ace-EFc）对子宫颈癌细胞，以及其对过氧化氢（H ₂ O ₂）诱导的非肿瘤HaCaT细胞脂过氧化和细胞毒性的保护作用。使用DPPH和ABTS自由基测定抗氧化活性。用MTT和1-甲基-2-苯基吲哚测定法分别测定细胞活力和脂过氧化。H ₂ O _2的模型在HaCaT细胞中诱导的细胞毒性和氧化损伤得以建立。在暴露于H ₂ O ₂之前或之后，将HaCaT细胞暴露于提取物中，并评估其氧化损伤和细胞活力。Ace-EFc抑制DPPH和ABTS自由基，并显示出对SiHa和HeLa细胞的细胞毒性作用。此外，提取物处理对过氧化氢诱导的脂质过氧化和细胞毒性具有保护作用，避免了MalonDiAldehyde（MDA）水平的增加和细胞生存力的下降（p <0.001）。这些结果表明，番红花代谢产物叶片具有抗氧化和抗氧化损伤的细胞保护活性。因此，它们对于保护细胞免受引起氧化应激的条件可能是有用的。