The study of vertebrate genome evolution is currently facing a revolution, brought about by next generation sequencing technologies that allow researchers to produce nearly complete and error-free genome assemblies. Novel approaches however do not always provide a direct link with information on vertebrate genome evolution gained from cytogenetic approaches. It is useful to preserve and link cytogenetic data with novel genomic discoveries. Sequencing of DNA from single isolated chromosomes (ChromSeq) is an elegant approach to determine the chromosome content and assign genome assemblies to chromosomes, thus bridging the gap between cytogenetics and genomics. The aim of this paper is to describe how ChromSeq can support the study of vertebrate genome evolution and how it can help link cytogenetic and genomic data. We show key examples of ChromSeq application in the refinement of vertebrate genome assemblies and in the study of vertebrate chromosome and karyotype evolution. We also provide a general overview of the approach and a concrete example of genome refinement using this method in the species Anolis carolinensis.

中文翻译：

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通过单染色体测序 (ChromSeq) 弥合脊椎动物细胞遗传学和基因组学之间的差距


脊椎动物基因组进化的研究目前正面临一场革命，这场革命是由下一代测序技术带来的，该技术使研究人员能够产生几乎完整且无错误的基因组组装。然而，新方法并不总是提供与通过细胞遗传学方法获得的脊椎动物基因组进化信息的直接联系。保存细胞遗传学数据并将其与新的基因组发现联系起来非常有用。对单个分离染色体进行 DNA 测序 (ChromSeq) 是一种确定染色体内容并将基因组组件分配给染色体的优雅方法，从而弥合了细胞遗传学和基因组学之间的差距。本文的目的是描述 ChromSeq 如何支持脊椎动物基因组进化研究以及它如何帮助连接细胞遗传学和基因组数据。我们展示了 ChromSeq 在脊椎动物基因组组装精化以及脊椎动物染色体和核型进化研究中应用的关键示例。我们还提供了该方法的总体概述以及使用该方法在 Anolis carolinensis 物种中进行基因组精炼的具体示例。