当前位置: X-MOL 学术ChemRxiv › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Detection of Metabolite-Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Towards Interactomics by NMR
ChemRxiv Pub Date : 2021-01-19
Ziqing Wang, Veronica Ghini, Pavel Kaderavek, Simone Pisano, Milan Zachrdla, Philippe Pelupessy, Morgan Kazmierczak, Thorsten Marquardsen, Jean-Max Tyburn, Guillaume Bouvignies, Giacomo Parigi, Claudio Luchinat, Fabien Ferrage

In this manuscript, we introduce an NMR method to investigate the interaction of metabolites and other small molecules with proteins in biological fluids. Our approach, based on high-resolution relaxometry identifies the interaction of small molecules with proteins in blood serum and is used for the quantitative analysis of binding competition in situ.

中文翻译:

高分辨率弛豫法检测复杂生物样品中代谢产物-蛋白质相互作用:通过NMR实现相互作用

在本文中,我们介绍了一种NMR方法来研究代谢物和其他小分子与生物流体中蛋白质的相互作用。我们基于高分辨率弛豫法的方法可识别小分子与血清中蛋白质的相互作用,并用于定量分析原位结合竞争。
更新日期:2021-01-19
down
wechat
bug