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Development and validation of an LC-MS/MS screening method for macrolide and quinolone residues in baby food
Journal of Environmental Science and Health, Part B ( IF 1.4 ) Pub Date : 2021-01-19 , DOI: 10.1080/03601234.2021.1872324
Jônatas V. Grutes 1 , Rosana G. Ferreira 1 , Mararlene U. Pereira 1 , Felipe S. Candido 1 , Bernardete F. Spisso 1
Affiliation  

Abstract

A simple, rapid and sensitive screening method by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the identification of 7 macrolides (clarithromycin, erythromycin, oleandomycin, spiramycin, tilmicosin, troleandomycin and tylosin) and 8 quinolones (ciprofloxacin, difloxacin, enrofloxacin, flumequine, moxifloxacin, nalidixic acid, norfloxacin and ofloxacin) in meat and egg-based baby foods. Sample preparation was performed using an alkaline modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction method without additional clean-up steps. A simplex-lattice mixture experimental design was used in the optimization of the QuEChERS extraction solvent. The developed method was successfully validated according to the Commission Decision 2002/657/EC and the European Community Reference Laboratories Residues Guidelines regarding the validation of screening methods 20/01/2010, adopting a fixed permited tolerance for relative ion ratio. Samples of baby food (n = 44) commercialized in Rio de Janeiro, Brazil, were analyzed using the validated method and none of them presented residues of the searched macrolides and quinolones, with a screening target value of 5 µg kg−1.



中文翻译:

婴儿食品中大环内酯和喹诺酮残留的LC-MS / MS筛选方法的开发和验证

抽象的

建立了一种简单,快速,灵敏的液相色谱-串联质谱(LC-MS / MS)筛选方法,用于鉴定7种大环内酯类(克拉霉素,红霉素,油橄榄霉素,螺旋霉素,替米考星,曲安霉素和泰乐菌素)和8种喹诺酮类药物(环丙沙星) ,肉类和鸡蛋类婴儿食品中的,地氟沙星,恩诺沙星,氟美卡因,莫西沙星,萘啶酸,诺氟沙星和氧氟沙星)。使用碱性修饰的QuEChERS(快速,简便,廉价,有效,坚固,安全)提取方法进行样品制备,而无需其他清理步骤。在QuEChERS萃取溶剂的优化中使用了单纯形-晶格混合物实验设计。根据委员会决定2002/657 / EC和欧洲共同体参考实验室残留物准则,关于筛选方法的验证已成功验证了所开发的方法,采用相对离子比率的固定允许公差,方法为20/01/2010。使用经过验证的方法对在巴西里约热内卢商业化的婴儿食品样本(n = 44)进行了分析,没有一个样本显示出所搜索的大环内酯类和喹诺酮类药物的残留,筛查目标值为5 µg kg-1

更新日期:2021-03-09
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