CCS Chemistry ( IF 9.4 ) Pub Date : 2021-01-19 , DOI: 10.31635/ccschem.020.202000501 Rui-Ying Guo 1 , Yu-Tian Zhang 2, 3 , Supphachok Chanmungkalakul 4 , Hao-Ran Guo 2, 3 , Yongzhou Hu 1 , Jia Li 2, 3, 5 , Xiaogang Liu 4 , Yi Zang 2 , Xin Li 1
Nitric oxide (NO) participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology. While there are methods available for detecting biological NO, few are capable of tracking NO dynamics. Herein, inspired by the cellular machinery of reversible thiol modification by NO, we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics. Biocompatible probes
中文翻译:
可逆荧光探针在活细胞中追踪一氧化氮动力学的生物启发设计
一氧化氮(NO)参与各种途径,揭示其动力学对解决其病理生理学至关重要。尽管存在用于检测生物NO的方法,但很少能够跟踪NO动态。在此,受NO可逆硫醇修饰的细胞机制的启发,我们成功设计了标记有荧光团的半胱氨酸类似物家族,用于可视化细胞NO动力学。生物相容性探针