The objectives were: 1. to investigate the occurrence of equine asthma syndrome (EAS) in a group of Campolina and Mangalarga Marchador horses through post-exercise respiratory endoscopy and cytology; 2. compare the efficiency of different diagnostic criteria; and 3. compare the incidence of EAS by breed, age group and sex. A standardised exercise test was conducted in the field, followed by respiratory endoscopy and bronchoalveolar lavage and fluid collection 30 min post-exercise. Clinical evaluation pre- and post-exercise, respiratory endoscopy post-exercise and bronchoalveolar lavage, also post-exercise, were conducted in 30 horses (15 Campolina and 15 Mangalarga Marchador) aged 3–18 years, without a history of respiratory illness and without clinical evidence of active respiratory disease. All horses were in active training and were considered fit to participate in equestrian competition characteristic of these breeds. Post-collection, the horses were subdivided into healthy or EAS-positive, depending on several diagnostic criteria. Statistical comparisons between breeds, age brackets (up to 7 years of age and above 7 years of age) and sex were conducted using appropriate statistical tests. According to bronchoalveolar lavage fluid (BALF) cytology, 56.7% of the horses showed at least one inclusion criteria that contributed to classification as EAS-positive. Of those, only one was classified as moderate EAS, while the others were classified as mild. Total cell count in the BALF above 530 cells/µL was the single most common diagnostic criteria (100%), followed by increased tracheal mucus (53%) at endoscopy, eosinophilia (47%) and neutrophilia (35%) in BALF. Significant differences were found between macrophage and lymphocyte numbers according to breed, but no other differences between breeds, age brackets, sex or status (healthy vs. EAS-positive). A high occurrence of EAS was found in competition-ready Mangalarga and Campolina pacer horses in this study.

中文翻译：

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无症状巴西起搏器马哮喘综合征的运动后内镜和细胞学诊断

目的是： 1. 通过运动后呼吸道内窥镜检查和细胞学检查一组 Campolina 和 Mangalarga Marchador 马的马哮喘综合征（EAS）的发生情况；2.比较不同诊断标准的效率；3. 按品种、年龄组和性别比较EAS的发病率。在现场进行了标准化运动测试，然后在运动后 30 分钟进行呼吸内窥镜检查和支气管肺泡灌洗和液体收集。对 30 匹马（15 匹 Campolina 和 15 匹 Mangalarga Marchador）进行了运动前和运动后的临床评估、运动后的呼吸内窥镜检查和支气管肺泡灌洗以及运动后的评估，这些马年龄在 3-18 岁之间，没有呼吸系统疾病史，也没有活动性呼吸道疾病的临床证据。所有马匹都在积极训练，被认为适合参加这些品种的马术比赛。收集后，根据几个诊断标准将马细分为健康或 EAS 阳性。使用适当的统计检验进行品种、年龄组（7 岁以下和 7 岁以上）和性别之间的统计比较。根据支气管肺泡灌洗液 (BALF) 细胞学，56.7% 的马显示出至少一项有助于分类为 EAS 阳性的纳入标准。其中，只有一个被归类为中度 EAS，而其他被归类为轻度。BALF 中的总细胞计数高于 530 个细胞/μL 是最常见的单一诊断标准 (100%)，其次是内镜检查时气管粘液增加 (53%)，BALF 中的嗜酸性粒细胞增多 (47%) 和中性粒细胞增多 (35%)。根据品种，巨噬细胞和淋巴细胞数量之间存在显着差异，但品种、年龄段、性别或状态（健康与 EAS 阳性）之间没有其他差异。在这项研究中，在准备比赛的 Mangalarga 和 Campolina 起搏马中发现了 EAS 的高发生率。