Adventitious roots (ARs) are produced from non‐root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, in a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation in young seedlings of Solanum lycopersicum cv. ‘Micro‐Tom’ after whole root excision by means of physiological, genetic and molecular approaches. We found that a regulated basipetal auxin transport from the shoot and local auxin biosynthesis triggered by wounding are both required for the re‐establishment of internal auxin gradients within the vasculature. This promotes cell proliferation at the distal cambium near the wound in well‐defined positions of the basal hypocotyl and during a narrow developmental window. In addition, a pre‐established pattern of differential auxin responses along the apical‐basal axis of the hypocotyl and an as of yet unknown cell‐autonomous inhibitory pathway contribute to the temporal and spatial patterning of the newly formed ARs on isolated hypocotyl explants. Our work provides an experimental outline for the dissection of wound‐induced AR formation in tomato, a species that is suitable for molecular identification of gene regulatory networks via forward and reverse genetics approaches.

中文翻译：

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番茄芽外植体伤口诱导不定根形成的生长素介导的调控框架

不定根 (ARs) 是从非根组织中产生的，以响应不同的环境信号，例如非生物胁迫或受伤后，在需要激素串扰的复杂发育过程中。在这里，我们表征了茄属植物幼苗中 AR 的形成简历。通过生理、遗传和分子方法进行全根切除后的“Micro-Tom”。我们发现，重新建立脉管系统内的内部生长素梯度都需要来自枝条的受调节的基叶生长素转运和由受伤引发的局部生长素生物合成。这在基底下胚轴的明确位置和狭窄的发育窗口期间促进了伤口附近远端形成层的细胞增殖。此外，沿着下胚轴顶端-基底轴的预先建立的不同生长素反应模式和未知的细胞自主抑制途径有助于分离下胚轴外植体上新形成的 AR 的时空模式。