Anti‐colon cancer activity tracking isolation of peptide from ginseng leaves and potential mechanisms evaluation in vitro and in vivo,Journal of Peptide Science

当前位置： X-MOL 学术 › J. Pept. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Anti‐colon cancer activity tracking isolation of peptide from ginseng leaves and potential mechanisms evaluation in vitro and in vivo
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2021-01-18 , DOI: 10.1002/psc.3297
Zhuo Liu ₁ , Xiaolei Liu ₂ , Wei Li ₂ , Qiang Luo ₃ , Jie Liu ₃ , Dongxin Wang ₄

Affiliation

The ginseng has been used for over hundred years, in the belief of promoting longevity. However, the anticancer activity of ginseng leaf peptide (GP) has been never explored. In current study, we isolated the GPs and explored the anti‐colon cancer activity in vitro and in vivo. MTT results showed that the GP‐1 (GP‐1~FKEHGY) performed most antiproliferative activity against colon cancer CT‐26 cells with an IC₅₀ of 86.4 ± 9.46 μM (48 h). Further study indicated that GP‐1 activated the caspases, regulated the p53/murine double minute 2 (MDM2) state, and induced the CT‐26 cells apoptosis in a mitochondrial pathway. Meanwhile, the GP‐1 arrested the CT‐26 cells in G0/G1 phase accompanied with cyclin expression regulation. In addition, GP‐1 significantly suppressed the tumor growth and induced the tumor cells apoptosis in vivo. Notably, the GP‐1 would be a potential anti‐colon cancer candidate.

中文翻译：

人参叶多肽的抗结肠癌活性追踪分离及体内外潜在机制评估

人参已被使用了一百多年，以促进长寿的信念。然而，人参叶肽（GP）的抗癌活性从未被探索过。在目前的研究中，我们分离了 GPs 并探索了体外和体内的抗结肠癌活性。MTT 结果显示 GP-1 (GP-1~FKEHGY) 对结肠癌 CT-26 细胞具有最大的抗增殖活性，IC ₅₀86.4 ± 9.46 μM（48 小时）。进一步研究表明，GP-1 激活半胱天冬酶，调节 p53/鼠双分钟 2 (MDM2) 状态，并通过线粒体途径诱导 CT-26 细胞凋亡。同时，GP-1 在 G0/G1 期阻滞 CT-26 细胞，并伴随细胞周期蛋白表达调节。此外，GP-1在体内显着抑制肿瘤生长并诱导肿瘤细胞凋亡。值得注意的是，GP-1 将成为潜在的抗结肠癌候选药物。

更新日期：2021-03-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11