The search for novel bioactive compounds and the identification of known ones in the plant kingdom are a challenge for the scientists working in different fields of plant science. In the recent years, mass spectrometry is the most frequently applied method for analysis of complex mixtures of plant metabolites. Twenty‐two alkaloids of different lycorine skeleton subtypes (with a Δ^3,4 double bond, with a Δ^4,11 double bond, with saturated rings C and D, and with aromatic ring C) were subjected to separation with gas chromatography and studied with electron impact mass spectrometry including single quadropole (GC‐EIMS), tandem mass (GC‐EIMS/MS) and high resolution mass (EI‐HRMS) detectors in order to determine their fragmentation pattern. The compounds showed excellent separation and specific MS fragmentation allowing structural determination. The GC–MS can be successfully applied for searching new and identification of known bioactive molecules, chemotaxonomical and chemoecological studies, among others, within the Amaryllidoideae subfamily.

中文翻译：

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一些lycorine型金石科生物碱的GC-MS

在植物界寻找新的生物活性化合物和鉴定已知的生物活性化合物，对从事植物科学不同领域的科学家构成了挑战。近年来，质谱法是分析植物代谢物复杂混合物的最常用方法。二十二个不同的生物碱石蒜碱骨架亚型（具有Δ的^3,4-双键，具有Δ ^4,11带有饱和环C和D以及带有芳香环C的双键）进行了气相色谱分离，并进行了电子碰撞质谱分析，包括单四极杆（GC-EIMS），串联质谱（GC-EIMS / MS）和高分辨率质量（EI-HRMS）检测器，以确定其碎裂模式。这些化合物表现出出色的分离度和特定的MS片段，可进行结构测定。GC-MS可以成功地用于在Amaryllidoideae亚科中搜索新的和鉴定的已知生物活性分子，化学分类学和化学生态学研究等。