Dairy byproducts represent a rich source of phospholipids with the potential for isolation and further commercialization. In this study, we extracted phospholipids from two dairy byproducts (buttermilk and beta-serum) using a tertiary amine (N,N-dimethylcyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent. For comparison, the phospholipids were extracted via Folch (chloroform: methanol). CyNMe2 extraction resulted in recovery values of 98.66 ± 0.89 and 7.67 ± 0.51% for buttermilk and beta-serum, respectively. Insights into the extraction mechanism were obtained by the analysis of protein profile, particle size, zeta potential, and microstructure. CyNMe2 generated microfractures and hollow openings in the solid matrix through ion pair formation that releases the phospholipids from the solid matrix. In comparison to Folch, CyNMe2 resulted in higher concentration of phospholipids (up to 9-fold increment), and it provided a different relative distribution, where phosphatidylcholine and phosphatidylinositol were the predominant phospholipids. The outcomes of this study help to gain insights into the extraction mechanism by which CyNMe2 acts, and develop extraction strategies for dairy byproducts.

乳制品副产品代表着丰富的磷脂来源，具有分离和进一步商业化的潜力。在这项研究中，我们使用叔胺（N，N-二甲基环己胺，CyNMe2）作为可切换的亲水性溶剂，从两种乳制品副产品（酪乳和β-血清）中提取了磷脂。为了比较，通过Folch（氯仿：甲醇）提取磷脂。CyNMe2提取对酪乳和β-血清的回收率分别为98.66±0.89和7.67±0.51％。通过分析蛋白质谱，粒度，ζ电势和微观结构，可以了解提取机理。CyNMe2通过离子对形成在固体基质中产生微裂缝和空心开口，离子对从固体基质中释放出磷脂。与福尔奇相比，CyNMe2导致较高的磷脂浓度（最多增加9倍），并且它提供了不同的相对分布，其中磷脂酰胆碱和磷脂酰肌醇是主要的磷脂。这项研究的结果有助于深入了解CyNMe2发挥作用的提取机制，并开发乳制品副产物的提取策略。