Plant-associated microbial communities form complex co-associations that play a role in promoting plant productivity and health, although the underlying mechanisms have not been fully elucidated. The functional role of the microbiome provides a new perspective on how we can potentially utilize the microbiome to maintain the postharvest quality and health of produce. While the composition and dynamics of the microbiome on fruit and vegetables over time and in response to postharvest management practices has begun to be explored, an understanding of their functional role in harvested produce is still lacking, especially in fruit. Therefore, the present study was undertaken to characterize the effect of near-harvest field application of a yeast biocontrol agent Metschnikowia fructicola, on the strawberry fruit microbiome. High-throughput sequencing revealed significant shifts in the bacterial and fungal community in response to the application of the yeast biocontrol agent sampled at the time of application, after harvest, and after storage and shelf life. Alterations included an increased bacterial diversity, distinct shifts in community composition and structure, specific microbial interactions and differential enrichment of several potentially beneficial genera (Methylobacterium, Sphingomonas, Rhizobium, Bacillus and others) in M. fructicola treated fruit leading to subsequent postharvest disease suppression. Results of this study provide new insights into the dynamics of the postharvest fruit microbiome that will assist in the development of a targeted, microbiome-driven approach to robust and sustainable disease control strategies.

与植物相关的微生物群落形成复杂的联合体，尽管其潜在机制尚未得到充分阐明，但它们在促进植物生产力和健康方面发挥着作用。微生物组的功能作用为我们如何潜在地利用微生物组保持采后质量和产品健康提供了新的视角。尽管已经开始探索水果和蔬菜上微生物组随时间的变化和动态以及对采后管理实践的反应，但仍缺乏对微生物在收获产品中的功能作用的了解，尤其是在水果中。因此，进行本研究以表征酵母生物防治剂Metschnikowia fructicola在近场田间的应用效果。，在草莓果实微生物组上。高通量测序揭示了响应于在施用时，收获后以及储藏和保存期限后采样的酵母生物控制剂的应用，细菌和真菌群落发生了显着变化。改变包括增加了细菌的多样性，群落组成和结构的明显变化，特定的微生物相互作用以及果蝇中几个潜在有益属（甲基杆菌，鞘氨醇单胞菌，根瘤菌，芽孢杆菌等）的差异富集。处理过的水果导致随后的采后病害抑制。这项研究的结果为收获后水果微生物组的动力学提供了新的见解，这将有助于开发针对性的，由微​​生物组驱动的方法，以制定稳健而可持续的疾病控制策略。