Uniform polyurea (PU) microspheres were prepared using isophorone diisocyanate as the only monomer either in binary solvents of water/acetone (H₂O/AT) or water/acetonitrile (H₂O/AN), or in ternary solvent of H₂O/AN/DMF, by precipitation polymerization without any shaking or stirring. Through careful examination, highly uniform microspheres were obtained at monomer concentration of 28.0 wt% at 65 °C in H₂O/AN at 20/80 by mass, a value much higher than the previously (13.0 wt%) achieved in H₂O/AT/DMF. The microspheres were analyzed by FTIR and NMR to confirm their PU structure and used as support for covalent immobilization of laccase via glutaraldehyde coupling. The immobilized laccase showed higher storage and thermal stability than the free laccase, and exhibited higher catalytic activity and better reusability in biodegradation of Congo Red and RBBR, compared with reported studies. In addition to enhancing the productivity of uniform microspheres, this study provides a new pathway to supported catalyst for enzymatic remediation of dyes-polluted water.

使用异佛尔酮二异氰酸酯作为唯一单体，在水/丙酮（H ₂ O / AT）或水/乙腈（H ₂ O / AN）的二元溶剂中或在H ₂ O的三元溶剂中，制备均匀的聚脲（PU）微球/ AN / DMF，通过沉淀聚合反应而无需任何摇动或搅拌。通过仔细检查，在65°C的H ₂ O / AN中以20/80质量计，单体浓度为28.0 wt％，获得了高度均匀的微球，该值大大高于先前在H _2中获得的值（13.0 wt％）O / AT / DMF。通过FTIR和NMR分析微球，以确认它们的PU结构，并用作通过戊二醛偶联共价​​固定漆酶的载体。与报道的研究相比，固定化的漆酶比游离漆酶具有更高的储存和热稳定性，并且在刚果红和RBBR的生物降解中表现出更高的催化活性和更好的可重复使用性。除了提高均匀微球的生产率外，这项研究还提供了一条新途径，可用于负载型催化剂的酶法修复染料污染的水。