Translationally controlled tumour proteins (TCTPs) are omnipresent in eukaryotes and play important physiological roles. This protein is identified as an allergen in the fungi Alternaria alternata and Cladosporium herbarum, and is involved in maintaining a balance between sexual and asexual differentiation in Aspergillus nidulans. MoTCTP regulates growth and conidiation in the plant pathogen Magnaporthe oryzae. We present here the functions of a TCTP orthologue (Tcp1) in the plant beneficial fungus Trichoderma virens. T. virens Tcp1 shares 42.94% and 39.88% sequence similarity with the evolutionarily distant human TCTP and maize TCTP, respectively. Based on prediction models, the secondary structure elements (α-helices and β-sheets) were found to be very well conserved barring a few insertions/deletions in the loop region. Using homologous recombination, we obtained three independent deletion mutants in this gene and a comparison of phenotypes with the wild type strain revealed that this protein has multiple functions in T. virens. Tcp1 knockout mutants showed slow radial growth and dry weight production. Δtcp1 mutants also lost the ability to overgrow the plant pathogenic fungi Sclerotium rolfsii and Rhizoctonia solani, but retained this property against the oomycete Pythium aphanidermatum reflecting selective loss of antagonistic ability. The mutants also lost the ability to colonize and kill the sclerotia of S. rolfsii.

翻译控制的肿瘤蛋白（TCTP）在真核生物中无处不在，并起着重要的生理作用。该蛋白被鉴定为真菌链格孢（Alternaria alternata）和阴枝菌（Cladosporium herbarum）中的变应原，并参与构巢曲霉中性与无性分化之间的平衡。MoTCTP调节植物病原体稻瘟病菌的生长和分生。我们在这里介绍TCTP直系同源基因（Tcp1）在植物有益真菌木霉中的功能。维尔纽斯Tcp1与进化上较远的人类TCTP和玉米TCTP分别具有42.94％和39.88％的序列相似性。根据预测模型，发现二级结构元素（α螺旋和β折叠）非常保守，但在环区域中只有一些插入/缺失。使用同源重组，我们在该基因中获得了三个独立的缺失突变体，并且与野生型菌株的表型比较表明该蛋白在T. virens中具有多种功能。Tcp1基因敲除突变体显示出缓慢的径向生长和干重生产。Δtcp1突变体也失去了植物致病真菌菌核病菌和茄根枯菌的过度生长能力，但保留了针对卵菌腐霉腐霉的这一特性，反映了拮抗能力的选择性丧失。突变体也失去了定殖和杀死罗氏链球菌菌核的能力。