In this work, a novel aptamer-based CA-MoS₂ hybrid biosensor was developed for diagnosis of Acute Myocardial Infraction (AMI). Specifically, a novel petal-like MoS₂ nanoflower was used as the active material on the Screen-Printed Electrode (SPE) for AMI biomarker determination. The aptamer-based CA-MoS₂–24 hybrid was able to attain a low limit of detection (LOD) and sensitivity to 10 fM at a linear range from 10 fM to 1 nM with good reproducibility based on an electrochemical impedance spectroscopy and a ~4 folds higher selectivity of troponin I compared to the signal from other biomolecules in human serum, retaining a 91% stability after 6 weeks. This novel CA-MoS2 aptamer-based biosensor with a 2.3% RSD value has the potential to revolutionize medical diagnosis by conducting multiple biomolecule detection on a single sensing platform.

在这项工作中，开发了一种新型的基于适体的CA-MoS ₂混合生物传感器，用于诊断急性心肌梗死（AMI）。具体而言，新型的花瓣状MoS ₂纳米花被用作丝网印刷电极（SPE）上的活性材料，用于AMI生物标志物的测定。基于适体的CA-MoS ₂–24杂化试剂能够在10 fM到1 nM的线性范围内达到低检测限（LOD）和对10 fM的灵敏度，基于电化学阻抗光谱法具有良好的重现性，并且与肌钙蛋白I相比，选择性提高了约4倍可以抵抗人血清中其他生物分子的信号，在6周后保持91％的稳定性。这种新颖的基于CA-MoS2适体的生物传感器，RSD值为2.3％，它有可能通过在单个传感平台上进行多个生物分子检测来彻底改变医学诊断。