Riptortus pedestris (Hemiptera: Alydidae) is a serious pest of soybean and sweet persimmon and uses male produced aggregation pheromone, (E)-2-hexenyl (Z)-3-hexenoate, (E)-2-hexenyl (E)-2-hexenoate, and tetradecyl isobutyrate to facilitate food location and recognition by conspecifics. Using electroantennogram (EAG) and greenhouse bioassay, we determined which antennal segment is involved in the detection of their aggregation pheromone. In the first EAG test using individual antennal segments, significant EAG responses to 1:1:1 mixture of the aggregation pheromone were observed only from the disti-flagellum segments of both male and female antennae at both pheromone doses tested (1 µg and 100 µg). In the following EAG tests using gradually removed antennal segment(s), EAG response was still maintained when the distal half of a disti-flagellum was surgically removed, while EAG response was lost when whole segment of disti-flagellum or other whole segments were gradually removed from intact antenna of both sexes. In greenhouse experiment, removing one or both segment(s) of disti-flagellum from male or female antennae resulted in significant reduction in their attraction to the aggregation pheromone. Together, these findings support that the disti-flagellum of R. pedestris houses olfactory neurons associated with attraction to their aggregation pheromone.

Riptortus pedestris（Hemiptera：Alydidae）是一种严重的大豆和甜柿害虫，使用雄性产生的聚集信息素，（E）-2-己烯基（Z）-3-己烯酸酯，（E）-2-己烯基（E）-2-己酸和异丁酸十四烷基酯，以利于食物的定位和特定物种的识别。我们使用电伏安图（EAG）和温室生物测定法，确定哪个触角段参与了其聚集信息素的检测。在使用单个触角段的第一个EAG测试中，仅在两种测得的信息素剂量（1 µg和100 µg）下，仅从雄性和雌性触角的双鞭毛段观察到对聚集信息素的1：EG显着EAG反应。 ）。在随后的使用逐渐去除的触角节段的EAG测试中，当通过手术去除双鞭毛的远端一半时，仍保持了EAG反应，而当逐渐分离整个鞭毛或其他整个鞭毛段时，EAG反应消失了。从两性完整的天线上移开。在温室实验中，从雄性或雌性触角中除去双鞭毛的一个或两个部分会导致它们对聚集信息素的吸引力显着降低。总之，这些发现支持了pedestris的房子嗅觉神经元与其聚集信息素的吸引力有关。