To explore the role of insulin and forkhead box protein O1 (FoxO1) on cell proliferation regulated by sirtuin 1 (Sirt1) in goose primary hepatocyte, goose primary hepatocytes were isolated and incubated with Sirt1 inhibitor and nicotinamide, and then, some hepatocytes were transfected with FoxO1 interference plasmid or treated with insulin. The indexes related with cell proliferation were measured. The results first showed Sirt1 inhibition induced by nicotinamide increased cell proliferation in goose primary hepatocytes (P < 0.05). Second, the cyclin D1 protein content of cotreatment of nicotinamide and miRNA-FoxO1 was higher than that of nicotinamide treatment (P < 0.05); rate of bromodeoxyuridine-positive cell of the cotreatment with nicotinamide and FoxO1 interference was between the single nicotinamide treatment and the single FoxO1 interference treatment (P < 0.05); there was no significant difference between nicotinamide treatment and cotreatment of nicotinamide and miRNA-FoxO1 in DNA synthesis rate (P > 0.05), protein content of p21 (P > 0.05), and cell proliferative index (PI) (67.40 vs. 69.00%). Finally, the PI of cotreatment with nicotinamide and insulin (56.65%) was lower than the single nicotinamide treatment (67.40%); the DNA synthesis rate in cotreatment of nicotinamide and insulin was lower than the single nicotinamide treatment (P < 0.05). These findings suggested that Sirt1 inhibition increased cell proliferation in goose primary hepatocytes, FoxO1 and insulin may play role in cell proliferation mediated by Sirt1 in goose primary hepatocyte; different influence is only observational, and further investigations are require to highlight the potential role of FoxO1 and insulin in cell proliferation mediated by Sirt1 in goose primary hepatocyte.

为了研究胰岛素和叉头盒蛋白O1（FoxO1）对鹅原代肝细胞中sirtuin 1（Sirt1）调控的细胞增殖的作用，分离了鹅原代肝细胞，并与Sirt1抑制剂和烟酰胺一起孵育，然后，将部分肝细胞转染FoxO1干扰质粒或用胰岛素处理。测量与细胞增殖有关的指标。结果首先表明，烟酰胺诱导的Sirt1抑制作用增强了鹅原代肝细胞的细胞增殖（P  <0.05）。其次，烟酰胺和miRNA-FoxO1共同处理的细胞周期蛋白D1蛋白含量高于烟酰胺处理（P <0.05）; 烟酰胺和FoxO1干扰联合处理的溴脱氧尿苷阳性细胞发生率在单次烟酰胺处理和单次FoxO1干扰处理之间（P  <0.05）；烟酰胺处理和烟酰胺与miRNA-FoxO1的共处理之间的DNA合成率（P  > 0.05），蛋白含量p21（P  > 0.05）和细胞增殖指数（PI）均无显着差异（67.40 vs. 69.00％） 。最终，烟酰胺和胰岛素共同治疗的PI（56.65％）低于单次烟酰胺治疗（67.40％）。烟酰胺和胰岛素共处理的DNA合成率低于单烟酰胺处理（P <0.05）。这些发现表明，Sirt1抑制作用增加了鹅原代肝细胞的细胞增殖，FoxO1和胰岛素可能在Sirt1介导的鹅原代肝细胞的细胞增殖中起作用。不同的影响只是观察性的，还需要进一步研究以突出FoxO1和胰岛素在鹅原代肝细胞中Sirt1介导的细胞增殖中的潜在作用。