Vibrio harveyi can cause infections and diseases in a variety of marine vertebrates and invertebrates, which is very harmful to the aquaculture industry. In this study, two phages were isolated and named VB_VhaP_Vh-5 (referred to as PVHp5) and VB_VhaM_VH-8 (referred to as PVHp8). Morphological analysis showed that PVHp5 belonged to Autographiviridae phage, while PVHp8 belonged to Myoviridae family. The results of genome analysis showed that PVHp5 had a double stranded DNA genome with a length of 42,485 bp and a G + C content of 44.48%, including 49 ORFs. PVHp8 also had a double stranded DNA genome with a length of 87,255 bp and a G + C content of 36.08%. It contained 150 ORFs and encodes two tRNAs, one of which was tRNA (pseudotrna). No genes related to drug resistance and virulence were detected in the two phage genomes. In vitro experiment, compared with the control group, the phage treatment group significantly inhibited the growth of V. harveyi VH5 (P < 0.05). Subsequently, we determined that the best effect was obtained when the feed was immersed in phage fermentation broth for 15 min as the phage adsorption and the buoyancy of the feed were significantly better at this time than at other times (P < 0.05). We also discussed the practicability of feeding phages as a route of administration to protect turbot (Scophthalmus maximus L.) from V. harveyi infection. The group whose MOI = 1 was significantly different from the other two phage treatment groups (MOI = 10, MOI = 100), which indicated that MOI = 10 and MOI = 100 could enhance the phage protection of V. harveyi VH5. There was no significant difference in weight gain, body length growth and feed conversion rate between the phage fed group and the control group (P > 0.05). Feeding phage cocktails may be another optimal therapeutic agent against V. harveyi infection in turbot culture.

哈维氏弧菌可在多种海洋脊椎动物和无脊椎动物中引起感染和疾病，这对水产养殖业非常有害。在这项研究中，分离出两个噬菌体，分别命名为VB_VhaP_Vh-5（称为PVHp5）和VB_VhaM_VH-8（称为PVHp8）。形态分析表明，PVHp5属于自噬病毒科噬菌体，而PVHp8属于肌病毒科。基因组分析结果表明，PVHp5具有一个双链DNA基因组，长度为42485 bp，G + C含量为44.48％，包括49个ORF。PVHp8还具有一个双链DNA基因组，长度为87,255 bp，G + C含量为36.08％。它包含150个ORF，并编码两个tRNA，其中一个是tRNA（pseudotrna）。在两个噬菌体基因组中未检测到与耐药性和毒力相关的基因。在体外实验中，与对照组相比，噬菌体治疗组显着抑制了哈维弧菌VH5的生长（P  <0.05）。随后，我们确定将饲料浸入噬菌体发酵液中15分钟可获得最佳效果，因为此时的噬菌体吸附和浮力明显好于其他时间（P  <0.05）。我们还讨论了喂养噬菌体作为保护大菱t（Scophthalmus maximus L.）免受哈氏弧菌侵害的给药途径的实用性感染。MOI = 1的组与其他两个噬菌体治疗组（MOI = 10，MOI = 100）显着不同，这表明MOI = 10和MOI = 100可以增强哈维弧菌VH5的噬菌体保护。噬菌体喂养组与对照组的体重增加，体长增长和饲料转化率之间无显着差异（P  > 0.05）。饲喂噬菌体混合物可能是对抗大菱culture培养中哈氏弧菌感染的另一种最佳治疗剂。