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Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey
Parasitology Research ( IF 1.8 ) Pub Date : 2021-01-19 , DOI: 10.1007/s00436-021-07050-w
Salih Macin , Serra Orsten , Rugıyya Samadzade , Bayram Colak , Hakan Cebeci , Duygu Fındık

Cystic echinococcosis is a neglected, zoonotic disease in Turkey. The disease is commonly seen in rural areas where the local population is in close contact with livestock and dogs. This research aimed to molecularly identify of hydatid cysts in cattle and human isolates from Konya, Turkey. Following sample collection, direct microscopy was performed. After direct examination, total DNA was extracted, and positive PCR products of cox 1 mitochondrial gene (~ 875 bp) were sequenced. A total of 83 hydatid cysts (cattle n = 57 and human n = 26), 82 were identified as Echinococcus granulosus sensu stricto (G1–G3 genotypes), and one human isolate was characterized as Echinococcus equinus (G4 genotype). Fertility rates of cysts belonging to cattle for liver and lung cysts were 93.3% and 80%, respectively. Out of 26 human originated isolates, 18 (69.2%) of cysts were found to be fertile. To the best of our knowledge, this is the first report of E. equinus from human host in Turkey.



中文翻译:

土耳其科尼亚的人和动物囊性棘球co虫病:分子鉴定和土耳其人宿主马马大肠杆菌的首次报道

在土耳其,囊性棘球co病是一种被忽视的人畜共患病。该病多见于农村地区,当地人口与牲畜和狗密切接触。这项研究旨在分子鉴定土耳其科尼亚的牛和人类分离物中的包虫囊肿。样品收集后,进行直接显微镜检查。直接检查后,提取总DNA,并对cox 1线粒体基因(〜875 bp)的阳性PCR产物进行测序。总共83个包虫囊肿(牛n = 57,人n = 26),其中82个被鉴定为细粒棘球E(G1-G3基因型),其中一个人类分离株的特征为马棘球characterized。(G4基因型)。牛囊肿的肝囊肿和肺囊肿的受精率分别为93.3%和80%。在26个人类起源的分离株中,发现18个(69.2%)的囊肿可育。据我们所知,这是土耳其人类寄主中的马马大肠杆菌的首次报道。

更新日期:2021-01-19
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