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Superradiative plasmonic nanoantenna biosensors enable sensitive immunoassay using the naked eye
Nanoscale ( IF 5.8 ) Pub Date : 2020-12-28 , DOI: 10.1039/d0nr06148d Yang Xiong 1, 2, 3, 4 , Tong Fu 2, 3, 5, 6, 7 , Daxiao Zhang 2, 3, 5, 6, 7 , Shunping Zhang 2, 3, 5, 6, 7 , Hongxing Xu 1, 2, 3, 4, 5
Nanoscale ( IF 5.8 ) Pub Date : 2020-12-28 , DOI: 10.1039/d0nr06148d Yang Xiong 1, 2, 3, 4 , Tong Fu 2, 3, 5, 6, 7 , Daxiao Zhang 2, 3, 5, 6, 7 , Shunping Zhang 2, 3, 5, 6, 7 , Hongxing Xu 1, 2, 3, 4, 5
Affiliation
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Easy-to-use and sensitive quantification of biomarkers has a great significance in disease prediction, diagnosis, and monitoring. Here, we report a biosensor for simple and sensitive biomarker detection based on the strong light scattering (brightness) of superradiative plasmonic nanoantennas. This nanoantenna is constructed using antibody-decorated gold nanoparticles (Au NPs) immobilized onto a gold mirror by the target antigen, forming a nanoparticle-on-mirror (NPOM) configuration. The NPOM produces an order of magnitude stronger light scattering in the red region compared with isolated Au NPs on the dielectric substrate, due to the strong near-field coupling of surface plasmons across the gap between the Au NPs and the gold film. The increased brightness allows one to observe the captured Au NPs with the naked eye using a dark-field optical microscope. The particle density of the Au NPs varies linearly with the concentration of the target antigen over a broad dynamic range from 10−3 to 103 ng mL−1. This dynamic range is three orders of magnitude broader than that obtained from the previous work based on a dark-field optical microscope. The limit of detection is 1 pg mL−1 (6.67 fM), which is three orders of magnitude more sensitive than that obtained in the previous work using similar conditions. The uniform spatial distribution of the Au NPs on the gold film was allowed to quantify biomarkers with a relative standard deviation as small as 1–7%. Biosensing using superradiative NPs can lower the detection limit, simplify, and speed up the detection procedure for biomarker detection.
中文翻译：
超辐射等离子体纳米天线生物传感器可使用肉眼进行灵敏的免疫测定
易于使用且灵敏的生物标志物定量在疾病预测，诊断和监测中具有重要意义。在这里，我们报告一种基于超辐射等离子体纳米天线的强光散射（亮度）的简单而灵敏的生物标志物检测的生物传感器。使用目标抗原将抗体修饰的金纳米颗粒（Au NPs）固定在金镜上，形成纳米镜上纳米颗粒（NPOM）构型，从而构建此纳米天线。与介电基片上的孤立Au NPs相比，NPOM在红色区域产生的光散射强度高一个数量级，这是由于表面等离激元在Au NPs和金膜之间的间隙上的强近场耦合。增加的亮度允许人们使用暗视野光学显微镜肉眼观察捕获的金纳米颗粒。Au NP的颗粒密度在10到10的宽动态范围内随目标抗原的浓度线性变化-3至10 3 ng mL -1。该动态范围比基于暗场光学显微镜的先前工作所获得的动态范围宽三个数量级。检测极限为1 pg mL -1（6.67 fM），比以前的工作在类似条件下获得的灵敏度高三个数量级。金膜上金纳米颗粒的均匀空间分布可以量化生物标志物，相对标准偏差小至1–7％。使用超辐射NP的生物传感可以降低检测限，简化并加快生物标记物检测的检测过程。
更新日期：2021-01-18
中文翻译：
超辐射等离子体纳米天线生物传感器可使用肉眼进行灵敏的免疫测定
易于使用且灵敏的生物标志物定量在疾病预测，诊断和监测中具有重要意义。在这里，我们报告一种基于超辐射等离子体纳米天线的强光散射（亮度）的简单而灵敏的生物标志物检测的生物传感器。使用目标抗原将抗体修饰的金纳米颗粒（Au NPs）固定在金镜上，形成纳米镜上纳米颗粒（NPOM）构型，从而构建此纳米天线。与介电基片上的孤立Au NPs相比，NPOM在红色区域产生的光散射强度高一个数量级，这是由于表面等离激元在Au NPs和金膜之间的间隙上的强近场耦合。增加的亮度允许人们使用暗视野光学显微镜肉眼观察捕获的金纳米颗粒。Au NP的颗粒密度在10到10的宽动态范围内随目标抗原的浓度线性变化-3至10 3 ng mL -1。该动态范围比基于暗场光学显微镜的先前工作所获得的动态范围宽三个数量级。检测极限为1 pg mL -1（6.67 fM），比以前的工作在类似条件下获得的灵敏度高三个数量级。金膜上金纳米颗粒的均匀空间分布可以量化生物标志物，相对标准偏差小至1–7％。使用超辐射NP的生物传感可以降低检测限，简化并加快生物标记物检测的检测过程。
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