Promoter engineering improves transcription efficiency in biomolecular assays,Chemical Communications

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Promoter engineering improves transcription efficiency in biomolecular assays
Chemical Communications ( IF 4.3 ) Pub Date : 2021-1-12 , DOI: 10.1039/d0cc07797f
Jisu Woo _{1,

2,

3,

4,

5} , Jung Ho Kim _{1,

2,

3,

4,

5} , Seokjoon Kim _{1,

2,

3,

4,

5} , Ki Soo Park _{1,

2,

3,

4,

5}

Affiliation

We identified a novel 12 bp promoter that significantly increased transcription efficiency. Unlike the standard 20 bp promoter, which contains both recognition and initiation regions, the new promoter contains only a recognition region and is more suitable for diagnostic applications due to its smaller size. This promoter effectively produced different light-up RNA aptamers via transcription. Moreover, we used the promoter to analyze RNase H activity and achieved a detection limit of 0.009 U mL⁻¹, which was significantly better than that achieved via previous methods. We propose that the new promoter may serve as a key component in various diagnostic applications.

中文翻译：

启动子工程提高了生物分子测定中的转录效率

我们确定了一个新的12 bp启动子，可以大大提高转录效率。与包含识别区和起始区的标准20 bp启动子不同，新启动子仅包含一个识别区，并且由于其较小的尺寸而更适合于诊断应用。该启动子通过转录有效地产生了不同的点亮RNA适体。此外，我们用的启动子来分析RNA酶H活性，并取得0.009ümL的检测限^-1，这是显著比取得了较好的通过以前的方法。我们建议新的启动子可以用作各种诊断应用程序中的关键组件。

更新日期：2021-01-18

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