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Promoter engineering improves transcription efficiency in biomolecular assays
Chemical Communications ( IF 4.3 ) Pub Date : 2021-1-12 , DOI: 10.1039/d0cc07797f
Jisu Woo 1, 2, 3, 4, 5 , Jung Ho Kim 1, 2, 3, 4, 5 , Seokjoon Kim 1, 2, 3, 4, 5 , Ki Soo Park 1, 2, 3, 4, 5
Affiliation  

We identified a novel 12 bp promoter that significantly increased transcription efficiency. Unlike the standard 20 bp promoter, which contains both recognition and initiation regions, the new promoter contains only a recognition region and is more suitable for diagnostic applications due to its smaller size. This promoter effectively produced different light-up RNA aptamers via transcription. Moreover, we used the promoter to analyze RNase H activity and achieved a detection limit of 0.009 U mL−1, which was significantly better than that achieved via previous methods. We propose that the new promoter may serve as a key component in various diagnostic applications.

中文翻译:

启动子工程提高了生物分子测定中的转录效率

我们确定了一个新的12 bp启动子,可以大大提高转录效率。与包含识别区和起始区的标准20 bp启动子不同,新启动子仅包含一个识别区,并且由于其较小的尺寸而更适合于诊断应用。该启动子通过转录有效地产生了不同的点亮RNA适体。此外,我们用的启动子来分析RNA酶H活性,并取得0.009ümL的检测限-1,这是显著比取得了较好的通过以前的方法。我们建议新的启动子可以用作各种诊断应用程序中的关键组件。
更新日期:2021-01-18
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