Colorimetric glucose sensing with multiple-color changes by using a MnO2 NSs–TMB nanosystem,Analytical Methods

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Colorimetric glucose sensing with multiple-color changes by using a MnO2 NSs–TMB nanosystem
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-22 , DOI: 10.1039/d0ay02184a
Rui Qian _{1,

2,

3,

4,

5} , Dan Gao _{1,

2,

3,

4,

5} , Liping Liu _{4,

5,

6,

7,

8} , Yuyang Jiang _{3,

5,

9,

10}

Affiliation

Glucose performs many essential functions associated with metabolic processes in the living system, and is closely related to many diseases such as diabetes and hypoglycemia. Most of the existing glucose concentration detection methods require complex instruments, which undoubtedly limit its widespread use. Here, we have designed a glucose colorimetric detection system composed of glucose, glucose oxidase (GOD), manganese dioxide nanosheets (MnO₂ NSs) and 3,3′,5,5′-tetramethylbenzidine (TMB) to achieve colorimetric detection with the naked eye. Compared with the single-color change of the colorimetric method in previous studies, multiple-color changes have been realized. MnO₂ NSs, as a kind of nanomaterial imitating oxidase, can directly oxidize TMB to oxTMB. Because oxTMB showed a dark yellow color when strongly oxidized and light blue when weakly oxidized, this feature can achieve multiple-color changes rather than a single-color change, which is helpful for colorimetric observation with the naked eye. Finally, we successfully used MnO₂ NSs for colorimetric detection of glucose and realized multiple-color changes, making it easier to achieve colorimetric observation with the naked eye. The linear detection range is 0–4000 μM and limit of detection is 5.0 μM. This is not only useful for glucose, but also has an important significance for other experiments considering colorimetric experiments with the naked eye.

中文翻译：

使用MnO2 NSs–TMB纳米系统进行比色葡萄糖感测，具有多种颜色变化

葡萄糖在生命系统中执行与代谢过程相关的许多基本功能，并且与许多疾病（例如糖尿病和低血糖症）密切相关。现有的大多数葡萄糖浓度检测方法都需要复杂的仪器，这无疑限制了它的广泛使用。在这里，我们设计了一种由葡萄糖，葡萄糖氧化酶（GOD），二氧化锰纳米片（MnO ₂ NSs）和3,3'，5,5'-四甲基联苯胺（TMB）组成的葡萄糖比色检测系统，以裸露的方式进行比色检测。眼。与以前的研究中比色法的单色变化相比，已经实现了多种颜色变化。的MnO ₂NSs作为一种模拟氧化酶的纳米材料，可以将TMB直接氧化为oxTMB。由于oxTMB在强氧化时显示深黄色，在弱氧化时显示淡蓝色，因此此功能可以实现多色变化，而不是单色变化，这有助于用肉眼进行比色观察。最终，我们成功地将MnO ₂ NSs用于葡萄糖的比色检测，并实现了多种颜色变化，从而更易于用肉眼观察比色观察。线性检测范围是0–4000μM，检测极限是5.0μM。这不仅对葡萄糖有用，而且对于其他考虑用肉眼进行比色实验的实验也具有重要意义。

更新日期：2021-01-18

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