The occurrence of hepatocellular carcinoma (HCC) is closely related to the chronic inflammation which caused liver fibrosis and cirrhosis, and the interaction between HCC and its microenvironment further drives tumorigenesis. However, the single-cell resolution in vivo study is lacking, which limits our molecular understanding of tumour biology in the liver. Here, using published single-cell sequencing technology (scRNA-seq) database, we analyzed the liver microenvironment at high resolution in an unbiased manner and demonstrated the transcriptomic comparison between various cell populations and subpopulations in HCC and cirrhosis tissues. We found that eight genes that are specifically expressed in the endothelial cell and stellate cell of the HCC patients and correlated them with their survival rate, which may provide novel diagnosis and treatment targets for the clinical application.

中文翻译：

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使用单细胞测序分析鉴定从肝硬化到肝细胞癌的不同基因表达特征

肝细胞癌（HCC）的发生与引起肝纤维化和肝硬化的慢性炎症密切相关，HCC与其微环境的相互作用进一步推动了肿瘤的发生。然而，体内单细胞分辨率缺乏研究，这限制了我们对肝脏肿瘤生物学的分子理解。在这里，我们使用已发表的单细胞测序技术 (scRNA-seq) 数据库，以无偏见的方式以高分辨率分析了肝脏微环境，并证明了 HCC 和肝硬化组织中各种细胞群和亚群之间的转录组学比较。我们发现8个基因在HCC患者的内皮细胞和星状细胞中特异表达并与其存活率相关联，这可能为临床应用提供新的诊断和治疗靶点。