Spatial metabolomics uses imaging mass spectrometry (IMS) to localize metabolites within tissue section. Here, we performed matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance-IMS (MALDI-FTICR-IMS) to identify the localization of asparaptine, a naturally occurring inhibitor of angiotensin-converting enzyme, in green spears of asparagus (Asparagus officinalis). Spatial metabolome data were acquired with an untargeted manner. Segmentation analysis using the data characterized tissue-type-dependent and -independent distribution patterns in cross-sections of asparagus spears. Moreover, asparaptine accumulated at high levels in developing lateral shoot tissues. Quantification of asparaptine in lateral shoots using liquid chromatography-tandem mass spectrometry (LC-MS/MS) validated the IMS analysis. These results provide valuable information for understanding the function of asparaptine in asparagus, and identify the lateral shoot as a potential region of interest for multiomic studies to examine gene-to-metabolite associations in asparaptine biosynthesis.

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使用成像质谱的空间代谢组学确定芦笋在芦笋中的定位

空间代谢组学使用成像质谱（IMS）来定位组织切片内的代谢产物。在这里，我们进行了基质辅助激光解吸/电离-傅立叶变换离子回旋共振-IMS（MALDI-FTICR-IMS）来鉴定芦笋（一种天然存在的血管紧张素转化酶抑制剂）在芦笋的绿色长矛（芦笋）中的定位厚朴）。空间代谢组数据以非靶向方式获得。使用该数据进行的细分分析表征了芦笋矛杆横截面中的组织类型依赖性和非依赖性分布模式。此外，天冬酰胺在发育的侧生芽组织中高水平积累。使用液相色谱-串联质谱法（LC-MS / MS）对侧枝中的天冬酰胺进行定量验证了IMS分析的有效性。这些结果为了解芦笋中芦笋的功能提供了有价值的信息，并确定侧枝为多组学研究中研究芦笋生物合成中基因与代谢物关联的潜在关注区域。