Decynium-22 (D-22) is an inhibitor of the uptake2 system of monoamine clearance, resulting in increased levels of dopamine, norepinephrine, and serotonin in the nervous system and elsewhere. Uptake2 is mediated by low-affinity, high-capacity transporters that are inhibited by glucocorticoids, suggesting a mechanism of fast glugocorticoid-monoamine interaction in the brain. D-22 dose-dependently increased anxiety-like behavior in adult zebrafish exposed to the light/dark test, monotonically increasing scototaxis (dark preference), but affecting risk assessment with an inverted-U-shaped response. These results suggest that the uptake2 system has a role in defensive behavior in zebrafish, presenting a novel mechanism by which stress and glucocorticoids could produce fast neurobehavioral adjustments in vertebrates. Data: https://github.com/lanec-unifesspa/decynium22

中文翻译：

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Decynium-22影响斑马鱼明暗测试中的行为

Decynium-22（D-22）是单胺清除摄取系统的抑制剂，可导致神经系统及其他部位的多巴胺，去甲肾上腺素和血清素水平升高。摄取2是由糖皮质激素抑制的低亲和力，高容量转运蛋白介导的，提示了大脑中糖皮质激素-单胺快速相互作用的机制。在暴露于明/暗测试的成年斑马鱼中，D-22剂量依赖性地增加了焦虑样行为，单调增加了苏格兰直轴性（暗偏好），但以倒U型反应影响了风险评估。这些结果表明，uptake2系统在斑马鱼的防御行为中起作用，提出了一种新的机制，应力和糖皮质激素可以通过这种机制在脊椎动物中产生快速的神经行为调节。数据：https：// github。