Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.

中文翻译：

---

暴露于法国万寿菊精油中的马铃薯的转录组分析，其重点是叶淀粉代谢和对科罗拉多马铃薯甲虫的防御

法国万寿菊的花条是马铃薯田中常用的驱虫剂。然而，法国万寿菊挥发物对马铃薯代谢，生理和诱导防御的作用尚不清楚。因此，进行了微阵列转录组分析以研究法国万寿菊精油（EO）对实验室种植的马铃薯的影响。暴露于EO 8小时后，采用气相色谱/质谱（GC / MS）检测到的萜品油烯和mon烯为主要化合物，与对照组相比，表达2796个转录物，其倍数变化> 2。略高数量的转录物抑制了表达（1493下调与1303上调）。由于标注到不同光合作用相关过程的成绩单大部分被下调，我们选择了10个参与叶淀粉代谢途径的基因，并使用定量逆转录聚合酶链反应（RT-qPCR）验证了微阵列模式。除了减少叶片中淀粉颗粒的合成和诱导的淀粉颗粒分解外，与受试马铃薯植株中的葡萄糖和果糖相比，长时间暴露于EO的8小时使蔗糖的积累略有增加。科罗拉多马铃薯甲虫的体外喂养生物测定表明，EO诱导的转录水平和糖代谢的交替引起喂养行为的增强和受试幼虫的整体发育。