Vibrio mimicus is an emerging pathogen, mainly associated with contaminated seafood consumption. However, little is known about its evolution, biodiversity, and pathogenic potential. This study analyzes the pan-, core, and accessory genomes of nine V. mimicus strains. The core genome yielded 2424 genes in chromosome I (ChI) and 822 genes in chromosome II (ChII), with an accessory genome comprising an average of 10.9% of the whole genome for ChI and 29% for ChII. Core genome phylogenetic trees were obtained, and V. mimicus ATCC-33654 strain was the closest to the outgroup in both chromosomes. Additionally, a phylogenetic study of eight conserved genes (ftsZ, gapA, gyrB, topA, rpoA, recA, mreB, and pyrH), including Vibrio cholerae, Vibrio parilis, Vibrio metoecus, and Vibrio caribbenthicus, clearly showed clade differentiation. The main virulence genes found in ChI corresponded with type I secretion proteins, extracellular components, flagellar proteins, and potential regulators, while, in ChII, the main categories were type-I secretion proteins, chemotaxis proteins, and antibiotic resistance proteins. The accessory genome was characterized by the presence of mobile elements and toxin encoding genes in both chromosomes. Based on the genome atlas, it was possible to characterize differential regions between strains. The pan-genome of V. mimicus encompassed 3539 genes for ChI and 2355 genes for ChII. These results give us an insight into the virulence and gene content of V. mimicus, as well as constitute the first approach to its diversity.

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拟态弧菌的核心和辅助基因组分析

拟弧菌是一种新兴病原体，主要与食用海鲜污染有关。但是，对其进化，生物多样性和致病性知之甚少。这项研究分析了九种拟态弧菌菌株的泛，核心和辅助基因组。核心基因组在染色体I（ChI）中产生了2424个基因，在染色体II（ChII）中产生了822个基因，其辅助基因组平均占整个基因组的10.9％（对于ChI）和29％（对于ChII）。获得了核心基因组系统树，并且在两个染色体中，模拟弧菌ATCC-33654菌株最接近外群。此外，系统进化研究了八个保守基因（fts Z，gap A，gyr B，topA，RPO A，REC A，MRE B，和PYR 1H），包括霍乱弧菌，弧菌parilis，弧菌metoecus，和弧菌caribbenthicus，清楚地表明进化枝分化。在ChI中发现的主要毒力基因与I型分泌蛋白，细胞外成分，鞭毛蛋白和潜在的调节剂相对应，而在ChII中，主要类别为I型分泌蛋白，趋化性蛋白和抗生素抗性蛋白。辅助基因组的特征是在两个染色体中均存在移动元件和毒素编码基因。基于基因组图谱，可以鉴定菌株之间的差异区域。的泛基因组拟态弧菌涵盖3539个基因志和2355个基因CHII。这些结果使我们深入了解拟南芥的毒力和基因含量，并构成了其多样性的第一种方法。