The aim of this review is to present the state-of-the art achievements reported in the last two decades in the field of pulsed laser deposition (PLD) of biocompatible calcium phosphate (CaP)-based coatings for medical implants, with an emphasis on their in vivo biological performances. There are studies in the dedicated literature on the in vivo testing of CaP-based coatings (especially hydroxyapatite, HA) synthesized by many physical vapor deposition methods, but only a few of them addressed the PLD technique. Therefore, a brief description of the PLD technique, along with some information on the currently used substrates for the synthesis of CaP-based structures, and a short presentation of the advantages of using various animal and human implant models will be provided. For an in-depth in vivo assessment of both synthetic and biological-derived CaP-based PLD coatings, a special attention will be dedicated to the results obtained by standardized and micro-radiographies, (micro) computed tomography and histomorphometry, tomodensitometry, histology, scanning and transmission electron microscopies, and mechanical testing. One main specific result of the in vivo analyzed studies is related to the demonstrated superior osseointegration characteristics of the metallic (generally Ti) implants functionalized with CaP-based coatings when compared to simple (control) Ti ones, which are considered as the “gold standard” for implantological applications. Thus, all such important in vivo outcomes were gathered, compiled and thoroughly discussed both to clearly understand the current status of this research domain, and to be able to advance perspectives of these synthetic and biological-derived CaP coatings for future clinical applications.

中文翻译：

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脉冲激光沉积制备的合成和生物衍生的磷酸钙基涂层的体内评估：综述。

这篇综述的目的是介绍近二十年来在用于医疗植入物的生物相容性磷酸钙（CaP）基涂层的脉冲激光沉积（PLD）领域中取得的最新成就，重点是它们的体内生物学性能。在专用文献中，有关于通过多种物理气相沉积方法合成的基于CaP的涂层（尤其是羟基磷灰石，HA）的体内测试的研究，但其中只有少数涉及PLD技术。因此，将提供PLD技术的简要说明，以及有关当前用于合成基于CaP的结构的底物的一些信息，并简要介绍使用各种动物和人类植入物模型的优势。为了对合成的和生物来源的基于CaP的PLD涂层进行深入的体内评估，将特别关注通过标准化和微型X射线照相，（计算机）X线断层摄影和组织形态学，变容光度法，组织学，扫描和透射电子显微镜，以及机械测试。体内分析研究的一项主要特定结果与经证实的用CaP基涂层功能化的金属（通常为Ti）植入物与简单的（对照）Ti相比具有优越的骨整合特性，后者被认为是“金标准”。 ”用于植入式应用。因此，收集，整理并彻底讨论了所有这些重要的体内结果，以清楚地了解该研究领域的当前状况，