The antioxidant, cytoprotective, and wound-healing potential of the essential oil from the resin of Pistacia lentiscus var. chia (mastic oil) was evaluated, along with that of its major components, myrcene and α-pinene. Antioxidant potential was monitored as: (i) direct antioxidant activity as assessed by 2,2-di-phenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ABTS assays; (ii) DNA damage protection activity; and (iii) cytoprotective activity as assessed via induction of transcription of genes related to the antioxidant response in human keratinocyte cells (HaCaT). The cytoprotective potential of the test substances was further evaluated against ultraviolet radiation B (UVB)- or H₂O₂-induced oxidative damage, whereas their regenerative capability was accessed by monitoring the wound closure rate in HaCaT. Μastic oil and major components did not show significant direct antioxidant activity, however they increased the mRNA levels of antioxidant response genes, suggesting indirect antioxidant activity. Treatment of HaCaT with the test substances before and after UVB irradiation resulted in increased cell viability in the cases of pre-treatment with mastic oil or post-treatment with myrcene. Increased cytoprotection was also observed in the case of cell treatment with mastic oil or its major components prior to H₂O₂ exposure. Finally, mastic oil and myrcene demonstrated a favorable dose-dependent effect for cell migration and wound closure. Collectively, mastic essential oil may exert its promising cytoprotective properties through indirect antioxidant mechanisms.

中文翻译：

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香精油黄连木变种的抗氧化和细胞保护潜力。嘉及其主要成分月桂烯和α-P烯

黄连木精油树脂精油的抗氧化，细胞保护和伤口愈合潜力。评估了chia（松香油）及其主要成分月桂烯和α- pine烯的含量。通过以下方式监测抗氧化潜力：（i）通过2,2-二苯基-1-吡啶并肼基（DPPH），2,2-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）和ABTS评估的直接抗氧化活性分析; （ii）DNA损伤保护活性；（iii）通过诱导与人类角质形成细胞（HaCaT）中抗氧化反应有关的基因的转录而评估的细胞保护活性。进一步评估了测试物质对紫外线B（UVB）-或H ₂ O的细胞保护潜力_2-诱导的氧化损伤，而其再生能力通过监测HaCaT中的伤口闭合率来获得。橄榄油和主要成分未显示出明显的直接抗氧化活性，但是它们增加了抗氧化反应基因的mRNA水平，表明具有间接抗氧化活性。在用乳香油进行预处理或用月桂烯进行后处理的情况下，在UVB辐照之前和之后用测试物质处理HaCaT可以提高细胞活力。在H ₂ O ₂之前用乳香油或其主要成分进行细胞处理的情况下，还观察到了细胞保护作用的增强。接触。最后，乳香油和月桂烯对细胞迁移和伤口闭合显示出良好的剂量依赖性作用。总体而言，乳香精油可通过间接抗氧化剂机制发挥其有希望的细胞保护特性。