ABSTRACT

The research aimed to evaluate the mechanical behaviour, hybridisation and osteoblast activities of novel baghdadite/PCL-graphene nanocomposite scaffold. The mechanical behaviour was examined via measuring the compressive strength and Young’s module, the hybridisation was evaluated by Fourier transform infrared spectroscopy, Raman spectroscopy, and Brunauer Emmet Teller, and the osteoblast activities were assessed via MG-63 osteoblast cells. The results rendered PCL as a significant factor to enhance the mechanical strength of ceramic scaffolds. Due to the existence of σ and π covalent bonds in its structure, hydrophilicity and biocompatibility, graphene could be applied in scaffolds’ chemical compound to greatly enhance their mechanical and biological behaviours. This scaffold indicated compressive strength and Young’s module higher than 2 MPa and 0.05 GPa. Regarding cell behaviours, MG-63 osteoblast cells spread and attached well on the scaffolds confirming the viability, cytotoxicity, excellent cell attachment and proliferation. The results indicated that this scaffold possesses outstanding potential as a temporary substrate for bone tissue engineering.

摘要

该研究旨在评估新型巴格达/ PCL-石墨烯纳米复合材料支架的力学行为，杂交和成骨活性。通过测量抗压强度和杨氏模量来检查机械行为，通过傅立叶变换红外光谱法，拉曼光谱法和布鲁瑙尔·艾米特·泰勒（Brunauer Emmet Teller）评估杂交，并通过MG-63成骨细胞评估成骨细胞活性。结果使PCL成为增强陶瓷支架机械强度的重要因素。由于其结构，亲水性和生物相容性中存在σ和π共价键，因此可以将石墨烯应用于支架的化合物中，从而大大增强其机械和生物学性能。该支架表明抗压强度和杨氏模量高于2 MPa和0.05 GPa。关于细胞行为，MG-63成骨细胞在支架上扩散并附着良好，证实了其活力，细胞毒性，出色的细胞附着力和增殖能力。结果表明，该支架具有作为骨组织工程的临时底物的突出潜力。