ABSTRACT

The present paper reports a magnesium oxide (MgO) nanoparticle incorporated carboxy methyl cellulose (CMC)/poly vinyl alcohol (PVA) composite film with favorable composition for skin tissue engineering application. CMC/PVA films were prepared with different polymer ratios and cross linked with glutaraldehyde. The films possess surface roughness favorable for cell adhesion, desired swelling behavior and controlled degradation. Among the films, CMC/PVA (50:50) have tensile strength (28.31 MPa) and hydrophilicity (69.8º contact angle) close to those of skin tissue. MgO nanoparticles was incorporated in CMC/PVA film to improve antimicrobial and cell supportive property. The MTT assay revealed that MgO–incorporated composite films were non-cytotoxic exhibiting higher cell viability which is favorable for cell proliferation. CMC/PVA films with 1.5% PVA showed higher cell viability than other composite films with desired antimicrobial property. Thus, CMC/PVA composite film containing 1.5% MgO was demonstrated to be a potential artificial extra cellular matrix for skin tissue engineering application.

摘要

本论文报道了一种含有氧化镁（MgO）纳米颗粒的羧甲基纤维素（CMC）/聚乙烯醇（PVA）复合薄膜，该薄膜具有适合皮肤组织工程应用的成分。CMC/PVA 薄膜以不同的聚合物比例制备并与戊二醛交联。该薄膜具有有利于细胞粘附的表面粗糙度、所需的溶胀行为和可控的降解。其中，CMC/PVA（50:50）的抗拉强度（28.31 MPa）和亲水性（69.8º接触角）接近于皮肤组织。将 MgO 纳米颗粒掺入 CMC/PVA 薄膜中以提高抗菌和细胞支持性能。MTT测定表明，掺入MgO的复合膜无细胞毒性，具有较高的细胞活力，有利于细胞增殖。1. CMC/PVA 薄膜。5% PVA 比其他具有所需抗菌性能的复合膜显示出更高的细胞活力。因此，含有 1.5% MgO 的 CMC/PVA 复合膜被证明是一种潜在的用于皮肤组织工程应用的人造细胞外基质。