ABSTRACT

DNA Topoisomerase II (TopoII) uses ATP hydrolysis to decatenate chromosomes so that sister chromatids can faithfully segregate in mitosis. When the TopoII enzyme cycle stalls due to failed ATP hydrolysis, the onset of anaphase is delayed, presumably to allow extra time for decatenation to be completed. Recent evidence revealed that, unlike the spindle assembly checkpoint, this TopoII checkpoint response requires Aurora B and Haspin kinases and is triggered by SUMOylation of the C-terminal domain of TopoII.

中文翻译：

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Aurora B 和 Haspin 激酶在中期拓扑异构酶 II 检查点中的作用

摘要

DNA 拓扑异构酶 II (TopoII) 使用 ATP 水解来分离染色体，以便姐妹染色单体可以在有丝分裂中忠实地分离。当由于 ATP 水解失败而导致 TopoII 酶循环停止时，后期的开始会延迟，大概是为了有额外的时间来完成 decatenation。最近的证据表明，与纺锤体装配检查点不同，这种 TopoII 检查点反应需要 Aurora B 和 Haspin 激酶，并且由 TopoII C 末端结构域的 SUMOylation 触发。