Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters,Plant Pathology

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Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters
Plant Pathology ( IF 2.3 ) Pub Date : 2021-01-18 , DOI: 10.1111/ppa.13341
Melina Simeone ₁ , Claudio Gómez ₂ , Ana Bertalmío ₃ , Esperanza Ruiz ₄ , Claudia Hauteville ₂ , Laura Godoy Suarez ₂ , Blas Tito ₂ , Maria Laura García ₁

Affiliation

Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.

中文翻译：

经诊断参数验证的RT-qPCR检测柑橘类牛皮癣病毒

柑橘类牛皮癣病毒（CPsV）是牛皮癣的重要病原体，是牛皮癣的重要病因。卫生和认证计划有助于减少许多柑橘种植地区因牛皮癣和其他可移植物传播的疾病所造成的疾病损害。对于检疫和认证计划，目前使用敏感指标植物上的生物索引（BI）诊断这些疾病中的大多数。对于柑橘类牛皮癣，可以通过分子方法（例如定量逆转录PCR（RT-qPCR））检测CPsV，该方法比BI便宜且快捷，但是到目前为止，这两种方法的灵敏度，可靠性和可重复性尚未进行比较。。在这项工作中，通过RT-qPCR分析使用BI和CPsV检测对来自阿根廷和乌拉圭的128株植物进行了分析。

更新日期：2021-01-18

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