Speciation driven by recombination in the evolution of tomato curly stunt virus in Mozambique,Plant Pathology

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Speciation driven by recombination in the evolution of tomato curly stunt virus in Mozambique
Plant Pathology ( IF 2.3 ) Pub Date : 2021-01-18 , DOI: 10.1111/ppa.13342
Osvaldo F.L. Sande _{1,

2} , Anelise F. Orílio ₁ , Baltazar A.I. Chipiringo _{1,

3} , César A.D. Xavier ₄ , F. Murilo Zerbini ₁

Affiliation

Begomoviruses constitute a group of viruses of great economic importance in agriculture, mainly in tropical and subtropical regions, including southern Africa. Tomato curly stunt virus (ToCSV) is an important begomovirus described in South Africa and later reported in Mozambique impacting tomato cultivation. In Mozambique, the severe disease caused by ToCSV limits tomato cultivation and compromises the income of small and medium farms. Due to the scarcity of information about this pathogen, in this study we explored its genetic variability to elucidate the mechanisms that drive its evolution. In 2019, 27 ToCSV full‐length sequences were obtained from tomato foliar samples with virus‐like symptoms collected in the district of Chokue in Gaza province, Mozambique. Sequence analysis showed that isolates from Mozambique have a nucleotide identity of 90.9%–96.9% with the isolate from South Africa (AF261885) and are grouped into two distinct strains that we named ToCSV‐Chok I and ToCSV‐Chok II. The ToCSV‐Chok II strain has a higher nucleotide diversity than ToCSV‐Chok I in the Rep gene. Recombination is involved in the evolutionary history of ToCSV, with both intraspecific and interspecific recombination events detected in the Rep gene. Our results indicate a prevalence of ToCSV in tomato crops in the Chokue district of Mozambique (the major tomato‐producing region of the country) and suggest that ToCSV is undergoing speciation due to the effect of recombination.

中文翻译：

重组推动莫桑比克番茄卷曲特技病毒进化的物种

牛瘟病毒是一类在农业中具有重要经济意义的病毒，主要在热带和亚热带地区，包括南部非洲。番茄卷曲特技病毒（ToCSV）是一种重要的bemomovirus，在南非有描述，后来在莫桑比克有报道，影响番茄种植。在莫桑比克，由ToCSV引起的严重疾病限制了番茄的种植，并损害了中小型农场的收入。由于缺乏有关该病原体的信息，因此在本研究中，我们探索了其遗传变异性，以阐明驱动其进化的机制。2019年，从莫桑比克加沙省乔库地区收集的具有病毒样症状的番茄叶样品中获得了27条ToCSV全长序列。序列分析表明，来自莫桑比克的分离物的核苷酸同一性为90。来自南非（AF261885）的分离株占9％–96.9％，并分为两个不同的菌株，我们将其命名为ToCSV-Chok I和ToCSV-Chok II。ToCSV-Chok II菌株在ToCSV-Chok II菌株中具有比ToCSV-Chok I更高的核苷酸多样性。Rep基因。重组参与ToCSV的进化历史，在Rep基因中检测到种内和种间重组事件。我们的结果表明，在莫桑比克（该国主要的番茄产区）的Chokue区，番茄作物中ToCSV的流行，并表明由于重组的影响，ToCSV正在形成物种。

更新日期：2021-01-18

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