Lithium chloride clinically used to treat mental diseases but it has some side effects like cognitive impairment, memory deficit. Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique that is able to change neural activity and gene transcription in the brain. The aim of the study is to provide a conceptual theoretical framework based on behavioral and molecular effects of tDCS on memory changes induced by lithium in male mice. we applied Anodal-tDCS and Cathodal-tDCS over the left PFC for 3 consecutive days tDCS for 20 min with 2 mA after injection of different doses of lithium/saline.Trained in fear condition and finally the day after that tested their memory persistency factors (freezing-latency) and other behavior such as grooming and rearing percentage time in the fear conditioning. P-mTOR/mTOR was analyzed using western blotting. The results obtained from the preliminary analysis of behavioral fear memory showed that lithium had destructive effect in higher doses and decreased freezing percentage time. However, both cathodal and anodal tDCS significantly improved memory and increased P-mTOR/mTOR level in the PFC. The results of this study indicate that cathodal and anodal tDCS upon the left prefrontal increased memory and reduced lithium side effects on memory consolidation and altered expression of plasticity-associated genes in the prefrontal cortex.

氯化锂临床上用于治疗精神疾病，但有认知障碍、记忆力减退等副作用。经颅直流电刺激 (tDCS) 是一种非侵入性脑刺激技术，能够改变大脑中的神经活动和基因转录。该研究的目的是提供一个基于 tDCS 对锂诱导雄性小鼠记忆变化的行为和分子影响的概念性理论框架。我们在左侧 PFC 上连续 3 天应用阳极-tDCS 和阴极-tDCS 20 分钟 tDCS，在注射不同剂量的锂/盐水后 2 mA。冻结延迟）和其他行为，例如在恐惧条件反射中梳理和饲养百分比时间。使用蛋白质印迹分析 P-mTOR/mTOR。从行为恐惧记忆的初步分析中获得的结果表明，锂在较高剂量下具有破坏作用，并减少了冻结时间。然而，阴极和阳极 tDCS 都显着改善了 PFC 中的记忆并增加了 P-mTOR/mTOR 水平。这项研究的结果表明，左前额叶的阴极和阳极 tDCS 增加了记忆，减少了锂对记忆巩固的副作用，并改变了前额叶皮层中可塑性相关基因的表达。阴极和阳极 tDCS 均显着改善了 PFC 中的记忆并增加了 P-mTOR/mTOR 水平。这项研究的结果表明，左前额叶的阴极和阳极 tDCS 增加了记忆，减少了锂对记忆巩固的副作用，并改变了前额叶皮层中可塑性相关基因的表达。阴极和阳极 tDCS 均显着改善了 PFC 中的记忆并增加了 P-mTOR/mTOR 水平。这项研究的结果表明，左前额叶的阴极和阳极 tDCS 增加了记忆，减少了锂对记忆巩固的副作用，并改变了前额叶皮层中可塑性相关基因的表达。