OBJECTIVE Changes in the partitioning of dissolved inorganic (DIC) and glucose were elucidated by utilising 13C labelled DIC or glucose, and quantifying the biochemical profile of mixotrophic, heterotrophic and photoautotrophic cultures of the microalga Tetraselmis suecica. RESULTS Mixotrophic cultivation increases microalgal productivity and changes their biochemical profile, due to an alteration in the partitioning of carbon within the cell. When cultured mixotrophically and heterotrophically, there is enhanced incorporation of carbon into shorter chain saturated fatty acids and non-lipid biomass, compared to photoautotrophic cultivation. Autotrophic culture results in increased total fatty acid content of cultures (4.19% dry weight compared to 2.13%) and shifts the fatty acid profile in favour of long-chain unsaturated fatty acids, such as 18:2 n-(9,12), compared to mixotrophic culture. Quantifying the changes in partitioning between DIC and glucose facilitates tailoring of the biochemical profile to develop "designer" algae. CONCLUSIONS There is a condition specific shift in carbon partitioning into different fatty acid and biochemical fractions in T. suecica, with more inorganic carbon partitioned into 18:2 n-(9,12) in photoautotrophic rather than mixotrophic cultures.

中文翻译：

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杂养、异养和光合自养培养物中葡萄糖和 DIC 的碳分配

目的 通过使用 13 C 标记的 DIC 或葡萄糖，并量化微藻 Tetraselmis suecica 的混养、异养和光合自养培养物的生化特征，阐明溶解无机物 (DIC) 和葡萄糖分配的变化。结果 由于细胞内碳分配的改变，混合营养培养提高了微藻的生产力并改变了它们的生化特征。与光能自养培养相比，在混合营养和异养培养时，碳向较短链饱和脂肪酸和非脂质生物量中的掺入增强。自养培养导致培养物的总脂肪酸含量增加（干重为 4.19%，而干重为 2.13%）并使脂肪酸分布有利于长链不饱和脂肪酸，例如 18：2 n-(9,12)，与混合营养培养相比。量化 DIC 和葡萄糖之间分配的变化有助于定制生化特征以开发“设计者”藻类。结论 在 T. suecica 中，碳分配到不同的脂肪酸和生化部分中存在条件特异性转变，更多的无机碳在光自养培养而不是混养培养中分配为 18:2 n-(9,12)。