A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan. Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%). Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa. DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively. DNA G + C content of strain NCCP-1664T was 70.0 mol%. Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids. The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp. nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).

中文翻译：

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Zafaria cholistanensis gen。十一月 sp. 11 月，从巴基斯坦 Cholistan 沙漠土壤中分离到的中等耐热和耐盐放线菌

从巴基斯坦乔利斯坦沙漠中分离出革兰氏染色阳性、非孢子形成、无色素和非运动性细菌，命名为 NCCP-1664T 菌株。NCCP-1664T 菌株的细胞是严格需氧的、过氧化氢酶阳性和氧化酶阴性的棒状球菌生长周期，可以在 pH 6.0-9.0（最佳 pH 7-8）、28-45°C（最佳 37°C）和可以耐受 0-16% NaCl（最佳 2%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 NCCP-1664T 属于微球菌科，与节杆菌属的成员相关，与银杏节杆菌 (98.9%)、A. halodurans (97.7%) 和 A. oryzae (97.1%) 和低于 97% 的其他相关分类群。发现菌株 NCCP-1664T 与上述类型菌株的 DNA-DNA 相关性值小于 54%，而米曲霉的数字 DDH 和平均核苷酸同一性 (ANI) 值分别为 20.9% 和 74.3%。NCCP-1664T 菌株的 DNA G + C 含量为 70.0 mol%。菌株NCCP-1664T的化学分类数据显示肽聚糖类型为A3αL-Lys-L-Ala；甲基萘醌作为 MK-9(H2) (67%)、MK-8(H2) (32%) 和 MK-7(H2) (1%)，主要脂肪酸作为反异 -C15:0 (51.2%)，反异-C17:0 (9.6%) 和 C18:1ω9c (6.9%) 和极性脂质分布，包括二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、二半乳糖二酰甘油、少量的单半乳糖二酰甘油、三甘露糖醇二酰甘油。系统发育分析以及化学分类数据、生理、生化特征允许将其描述为新属的代表，名称 Zafaria cholistanensis gen。十一月 sp. 十一月 建议使用类型菌株 NCCP-1664T (= DSM 29936T = KCTC 39549T)。