Gemcitabine (GEM) and its derivatives of deoxycytosine is a promising anticancer candidate which is effective for the treatment of various cancers including lung cancer via cascade targetting Erk/Mek/Raf/Ras pathway and blocking the proliferation of the tumor cells. In this present work, we have described reduced graphene oxide (rGO) in the presence of anticancer utilizing ascorbic acid as reducing agents for lung cancer treatment. GEM reduced graphene oxide (termed as GEM-rGO) has resulted in a smooth and transparent morphological surface, which was confirmed by various spectroscopical investigations. The anticancer drug-loaded rGO has displayed remarkable cytotoxic activities against a panel of lung cancer cell lines when compared to the untreated lung cancer cells. Further, we examined the morphological observation of the cancer cell death was monitored through the fluorescence microscopic examinations. In addition, the cell deaths of the lung cancer cells were observed by the flow cytometry analyses. In addition, the non-toxic nature of potent GEM-rGO and GEM-rGO + NIR was confirmed by in vivo systemic toxicity analysis. Besides, the higher safety feature of the GEM-rGO and GEM-rGO + NIR was evidenced by histological analyses of the mice organs. The subcutaneous injection of GEM-rGO and GEM-rGO + NIR into mice bearing A549 xenografts more effectively inhibited the tumor than the free GEM. Based on the outcomes, we can summarise that the GEM reduced graphene oxide (GEM-rGO) can be used as a promising drug candidate for the treatment of lung cancer in the future.

吉西他滨（GEM）及其脱氧胞嘧啶衍生物是一种有前途的抗癌候选药物，可通过靶向Erk / Mek / Raf / Ras途径并阻断肿瘤细胞的增殖，有效治疗包括肺癌在内的各种癌症。在本工作中，我们描述了在抗癌存在下利用抗坏血酸作为肺癌治疗的还原剂还原的氧化石墨烯（rGO）。GEM还原的氧化石墨烯（称为GEM-rGO）产生了光滑透明的形态表面，这已通过各种光谱研究得到了证实。与未处理的肺癌细胞相比，负载抗癌药物的rGO对一组肺癌细胞系表现出了显着的细胞毒性活性。进一步，我们通过荧光显微镜检查检查了癌细胞死亡的形态学观察。另外，通过流式细胞术分析观察到肺癌细胞的细胞死亡。此外，体内全身毒性分析证实了强效GEM-rGO和GEM-rGO + NIR的无毒性质。此外，通过对小鼠器官的组织学分析证明了GEM-rGO和GEM-rGO + NIR具有更高的安全性。与游离GEM相比，将GEM-rGO和GEM-rGO + NIR皮下注射到带有A549异种移植物的小鼠中可以更有效地抑制肿瘤。根据结果​​，我们可以总结出，GEM还原氧化石墨烯（GEM-rGO）可以用作将来治疗肺癌的有希望的药物。