Pre-sowing seed priming and seedling inoculation with bioelicitor improve growth and phytochemical constituents of medicinal plants. This study was aimed to investigate the role of seed priming with silicon nanoparticles (nSi, 0, 100 and 500 mg/L) and inoculation of seedling originated from primed seeds with rhizobacteria strains (Pseudomonas fluorescens and P. putida) on physiological and metabolic attributes of Melissa officinalis L. Foliar application of P. putida on plants raised from nSi-primed (at 500 mg/L) seeds showed the wider deposition of nSi on root surface compared with that of P. fluorescens, however, P. fluorescens application on plants grown from nSi-primed (at 100 mg/L) seeds showed more open stomata on leaf surface than non-inoculated controls. Individual treatments of seed priming and seedling inoculation significantly (P < 0.05) increased plant biomass indices, leaf relative water content, photosynthetic pigments values, total soluble protein and phenolic contents, essential oil yield and their all (except thymol) major constituents, neral, geranial, and geranyl acetate compared with the control. However, the maximum increase in measured traits was recorded in mixed treatment of seed priming and inoculation of plants raised from primed seeds with rhizobacteria. Furthermore, the employed treatment combinations enhanced the free radical scavenging activities of plant extracts compared with the individual treatment and untreated control samples. According to the multivariate analyses, the treatments applied in combinations (especially seed priming at 100 mg nSi/L and seedling inoculation with P. putida) scores were substantially farther from the other treatments mean. Overall, seed priming with nSi along with seedling inoculation with pseudomonas strains play vital role in the increase in both primary and secondary metabolites of lemon balm plants.

播种前的种子引发和用生物引发剂接种可改善药用植物的生长和植物化学成分。这项研究旨在研究硅纳米颗粒（n Si，0、100和500 mg / L）引发种子以及接种根瘤菌菌株（荧光假单胞菌和恶臭假单胞菌）引发的种子的幼苗在生理和代谢中的作用的属性香蜂草的L.叶面喷施恶臭假单胞菌上从凸起植物ñ硅引发的（500毫克/升）的种子显示出更广泛的沉积ñ硅根表与相比荧光假单胞菌，但是，在由n硅引发的（100 mg / L）种子生长的植物上施用荧光假单胞菌比未接种对照显示出更多的叶片气孔。种子处理和幼苗接种的个别处理效果显着（P<0.05）与对照相比，植物生物量指数，叶片相对水含量，光合色素值，总可溶性蛋白和酚含量，精油产量及其所有（百里香酚除外）主要成分，神经，香叶醛和乙酸香叶酯含量增加。但是，在混合处理种子引发和接种带根瘤菌的引发种子的植物的接种中，测得的性状最大增加。此外，与单独处理和未处理的对照样品相比，所采用的处理组合增强了植物提取物的自由基清除活性。根据多变量分析，这些处理组合使用（特别是在100 mg n Si / L的底漆接种和恶臭假单胞菌的接种）中。）得分与其他治疗方法的平均值差很多。总的来说，用n Si引发种子以及接种假单胞菌菌株的幼苗在柠檬香脂植物初级和次级代谢产物的增加中起着至关重要的作用。