Cytoplasmic dynein is a minus end-directed microtubule motor that can be activated by cargo adapters. In Aspergillus nidulans, overexpression of ΔC-HookA, the early endosomal adapter HookA missing its cargo-binding site, causes activated dynein to accumulate at septa and spindle pole bodies (SPBs) where the microtubule-organizing centers are located. Intriguingly, only some interphase nuclei show SPB signals of dynein. Here we present data demonstrating that localization of the activated dynein at SPBs is cell cycle-dependent: SPB dynein signals are seen to associate with nuclei at early G1 but disappear at about the G1-S boundary.

细胞质动力蛋白是一种负端定向微管马达，可由货物适配器激活。在构巢曲霉中，ΔC-HookA 的过表达，早期的内体适配器 HookA 缺少其货物结合位点，导致活化的动力蛋白在微管组织中心所在的隔膜和纺锤体极体 (SPB) 处积聚。有趣的是，只有一些间期细胞核显示动力蛋白的 SPB 信号。在这里，我们提供的数据表明，活化的动力蛋白在 SPBs 的定位是细胞周期依赖性的：SPB 动力蛋白信号在 G1 早期与细胞核相关，但在 G1-S 边界处消失。