Development of an effective biotechnological method of reducing the continuous emission of CO₂ has caused wide public concern. Research on serpentine bio-weathering coupled with carbonation is a current focus of interest. To investigate the molecular mechanisms, Bacillus subtilis was cultured in the Mg²⁺-free medium with the addition of serpentine. Based on the composition of organic matter and the characterization of the weathered serpentine, quantitative proteomics technology was adopted to elucidate the roles of proteins played in the coupled process. Electrochemical analysis and vapor diffusion crystallization experiments were also performed to reveal the molecular mechanisms. The results demonstrated that more oxidoreductases were secreted to facilitate the transformation of serpentine, and drive the synthesis of organic acids to accelerate the weathering of serpentine. In the late stage of serpentine weathering, that is, the serpentine carbonation stage, the characterization of the secondary minerals showed that struvite and amorphous carbonate had been formed with some organic matter. In serpentine carbonation stage, excessive Ni in the microenvironment drove N metabolism to facilitate the elevation of pH, resulting in the serpentine carbonation. This study enriched our understanding of the molecular mechanisms at protein-level in serpentine bio-weathering coupled with carbonation.

减少CO ₂连续排放的有效生物技术方法的发展引起了广泛的公众关注。蛇纹石生物风化与碳酸化的研究是当前关注的焦点。为了研究分子机制，枯草芽孢杆菌在Mg ^2+中培养无蛇纹石的培养基。基于有机物的组成和风化蛇纹石的表征，采用定量蛋白质组学技术来阐明蛋白质在偶联过程中的作用。还进行了电化学分析和蒸汽扩散结晶实验，以揭示其分子机理。结果表明，分泌更多的氧化还原酶以促进蛇纹石的转化，并驱动有机酸的合成以加速蛇纹石的风化。在蛇纹岩风化的后期，即蛇纹岩的碳酸盐化阶段，次生矿物的特征表明鸟粪石和无定形碳酸盐是由某些有机物形成的。在蛇形碳酸化阶段，在微环境中过量的镍驱动氮的代谢以促进pH的升高，从而导致蛇形碳酸化。这项研究丰富了我们对蛇纹石生物风化结合碳酸化过程中蛋白质水平的分子机制的理解。