Traditional Chinese medicine (TCM) formulas have a significant clinical efficacy, and the fingerprint technology has been widely accepted to fully reveal the quality of TCM. Whereas, it is a great challenge to establish the fingerprint chromatogram which can fully reflect every single herb material in a short time. In this study, we used Xiaojin capsule (XJC) as a case and developed a rapid fingerprint method based on increasing the column temperature and flow rate simultaneously combined with computer-aided. First, the elution gradient was optimized based on the retention parameters and peak shape parameters of the four linear gradients, and then, the column temperature and flow rate were increased simultaneously to shorten the analysis time. Next, the standard fingerprint chromatogram of XJC, which can reflect every herb material, was generated. Finally, quality markers were screened through unsupervised cluster analysis and supervised orthogonal partial least squares discrimination analysis. Combining computer-aided with increasing column temperature and flow rate simultaneously can develop the rapid method for establishing HPLC fingerprint of XJC, which can fully reflect every single herb material and provide comprehensive quality control. The strategy for establishing HPLC fingerprint of TCM formula could be applied to other traditional Chinese medicine formulas and herbal medicine.

中文翻译：

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高效液相色谱法快速建立小金胶囊指纹图谱

中药配方具有显着的临床疗效，指纹技术已被广泛接受以充分揭示中药的质量。然而，建立指纹色谱图以在短时间内完全反映每种单一草药材料是一个巨大的挑战。在本研究中，我们以小金胶囊（XJC）为例，并在提高色谱柱温度和流速的同时结合计算机辅助技术开发了一种快速指纹方法。首先，根据四个线性梯度的保留参数和峰形参数优化洗脱梯度，然后同时提高柱温和流速以缩短分析时间。接下来，生成了可以反映每种草药材料的XJC标准指纹图谱。最后，通过无监督聚类分析和有监督的正交偏最小二乘判别分析筛选质量标记。结合计算机辅助提高色谱柱温度和流速，可以开发出快速建立XJC HPLC指纹图谱的方法，该方法可以充分反映每种草药材料并提供全面的质量控制。建立中药配方HPLC指纹图谱的策略可以应用于其他中药配方和中草药。可以充分反映每种草药材料，并提供全面的质量控制。建立中药配方HPLC指纹图谱的策略可以应用于其他中药配方和中草药。可以充分反映每种草药材料，并提供全面的质量控制。建立中药配方HPLC指纹图谱的策略可以应用于其他中药配方和中草药。