PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq, and proteomic approaches, we show that Plasmodium PP1 is implicated in both mitotic exit and, potentially, establishing cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.

中文翻译：

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蛋白磷酸酶1调节疟原虫性阶段的非典型有丝分裂和减数分裂分裂

PP1是一种保守的真核丝氨酸/苏氨酸磷酸酶，可调节有丝分裂和减数分裂的许多方面，通常与其他磷酸酶（如CDC14和CDC25）协同作用。疟疾寄生虫生命周期的增生阶段包括蚊媒内的性发育，其特征是雄性配子的形成具有非典型的快速有丝分裂，包括三轮DNA合成，相继的纺锤体形成和成簇的动植物，以及在受精卵到合子期间的减数分裂阶段。受精后的机体发育。尚不清楚PP1如何参与这些异常过程。使用实时活细胞和超微结构成像，条件基因敲低，RNA序列和蛋白质组学方法，我们表明疟原虫PP1参与有丝分裂出口，并有可能，