Abstract

Classical Swine Fever (CSF) is a contagious viral disease of pigs which is endemic in several parts of the world, including India. Prophylactic vaccination using live attenuated vaccine is the preferred method of control. However, there is significant inter-individual variation in the antibody response to vaccination. In this study, we measured the E2 antibody blocking percentage after 21 days of CSF vaccination in a mixed pig population consisting of Landrace, indigenous Ghurrah pigs, and their crossbreds. A Genome Wide Association Study (GWAS) carried out using single-SNP and haplotype based methods detected a 1.6 Mb region on SSC2 (28.92−30.52 Mb) as significantly associated with antibody response to CSF vaccination. The significant region and 1 Mb flanking sequences encompass 3 genes – EIF3M, DNAJC24 and ARL14EP, which code for proteins involved in Pestivirus replication and host immune response system. Our results combined with previous studies on immune response of pigs present this region as a suitable candidate for future functional investigations.

摘要

古典猪瘟 (CSF) 是一种猪的传染性病毒病，在世界多个地区流行，包括印度。使用减毒活疫苗进行预防性接种是首选的控制方法。然而，对疫苗接种的抗体反应存在显着的个体差异。在这项研究中，我们测量了由长白猪、本土 Ghurrah 猪及其杂交种组成的混合猪群在接种 CSF 疫苗 21 天后的 E2 抗体阻断百分比。使用基于单 SNP 和单倍型的方法进行的全基因组关联研究 (GWAS) 检测到 SSC2 (28.92–30.52 Mb) 上的一个 1.6 Mb 区域与 CSF 疫苗接种的抗体反应显着相关。显着区域和 1 Mb 侧翼序列包含 3 个基因 – EIF3M，DNAJC24和ARL14EP，编码参与瘟病毒复制和宿主免疫反应系统的蛋白质。我们的结果与之前关于猪免疫反应的研究相结合，表明该区域是未来功能研究的合适候选者。