ABSTRACT

In this study, jute and flax mats were used as a support material to deposit bacterial cellulose (BC) on its surface produced by Gluconacetobacter liquefaciens (MTCC 3135) through fermentation process under static condition. Amount of BC produced during the study indicates that type of mats as support material affects the production of BC at the air-liquid interface. About 2.45 g/l and 1.85 g/l of BC was produced in presence of jute and flax mats as support, respectively. Neat BC obtained under static cultivation was found to be brittle in nature. In order to prevent brittleness in BC, mat hanging method has been proposed to deposit BC directly on the surface of the mat. FTIR peaks obtained at 3337 and 3350 cm⁻¹ as well as at 1647 and 1654 cm⁻¹ indicated the presence of cellulose in form of BC on both the mats. Microscopic observation, water uptake behavior and antibacterial studies of neat mats, unadhered BC and BC deposited on mats were also carried out and the results were compared. The proposed in-situ method of collecting BC on the cellulosic mats will help in fabrication of biocomposite sheets that can be further explored in packaging, medical and biomedical applications.

摘要

在这项研究中，黄麻垫和亚麻垫用作支撑材料，以在静态条件下通过发酵过程将液化葡糖杆菌（MTCC 3135）产生的细菌纤维素（BC）沉积在其表面上。在研究过程中产生的BC量表明，垫子作为支撑材料的类型会影响气液界面处BC的产生。在黄麻垫和亚麻垫作为载体的情况下，分别生产出约2.45 g / l和1.85 g / l的BC。发现在静态栽培下获得的纯BC在性质上是脆的。为了防止BC的脆性，已经提出了垫悬挂方法以将BC直接沉积在垫的表面上。在3337和3350 cm ^-1以及1647和1654 cm ^-1处获得的FTIR峰表明在两个垫子上都以BC形式存在纤维素。还对纯垫，未粘附的BC和沉积在垫上的BC进行了显微镜观察，吸水行为和抗菌研究，并将结果进行了比较。拟议的在纤维素垫上收集BC的原位方法将有助于制造生物复合片材，可在包装，医疗和生物医学应用中进一步探索。